Acupuncture Ameliorates Neuronal Cell Death, Inflammation, and Ferroptosis and Downregulated miR-23a-3p After Intracerebral Hemorrhage in Rats
Abstract
Baihui-penetrating-Qubin acupuncture is frequently used to treat intracerebral hemorrhage (ICH) in China. Acupuncture affects multiple microRNAs in diseases. MicroRNA-23a-3p (miR-23a-3p) has been demonstrated to be up-regulated in ICH patients. Herein, the effect of Baihui-penetrating-Qubin acupuncture on miR-23a-3p expression after ICH and the role of miR-23a-3p in ICH were discussed. A rat model of ICH was induced by infusing autologous blood into caudate nucleus. Acupuncture was performed after ICH once a day for 30 min. After 3 consecutive days of acupuncture, the neurobehavioral function, brain edema, neuronal cell death, inflammation, ferroptosis, nuclear factor E2-like 2 (NFE2L2) signaling and miR-23a-3p levels in brain tissues were analyzed. Additionally, antagomiR-23a-3p was injected into rats 3 days prior to ICH modeling to analyze the function of miR-23a-3p in neuronal cell death, inflammation, ferroptosis, and NFE2L2 signaling. Acupuncture relieved the ICH-induced neurological function deficits, increases in brain water content and Fluoro-Jade B (FJB)-positive cells and release of proinflammatory cytokines. Acupuncture also alleviated ferroptosis and decreased miR-23a-3p expression, as evidenced by the increased NFE2L2 nuclear translocation and expressions of heme oxygenase-1 and glutathione peroxidase 4 and the decreased iron and malondialdehyde contents and reactive oxygen species accumulation. Additionally, antagomiR-23a-3p inhibited the ICH-induced increases i…
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Acupuncture stimulation can down-regulate the expression of miR-23a-3p, thereby improving neurological dysfunction from intracerebral hemorrhage in rats, alleviating inflammation, ferroptosis, and neuronal cell death.
“acupuncture stimulation can down-regulate the expression of miR-23a-3p, thereby improving the neurological dysfunction stemming from intracerebral hemorrhage (ICH) in rats, alleviating inflammation, ferroptosis, and neuronal cell death [67].”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [67]
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References 63 · Cited by 55
- Authors
- Kong, Y., Li, S., Zhang, M., Xu, W., Chen, Q., Zheng, L., Liu, P., & Zou, W.
- Affiliations
- Heilongjiang University of Chinese Medicine (CN)
- Corresponding author
- Zou, Wei
- Venue
- Journal of Molecular Neuroscience
- Published
- 2021-01-05
- Volume
- 71
- Issue
- 9
- Pages
- 1863-1875
- ISSN
- 0895-8696 (ISSN-L)
- 1559-1166
- OpenAlex
- W3120647133
- Cited by
- 55
- References
- 63
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 15 · 2023: 7 · 2022: 9 · 2021: 4
- FWCI
- 9.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (No. 81904305)
- Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (No. 2017bs05)
- Heilongjiang Provincial Postdoctoral Science Foundation (No. LBH-Z12249)
- Project of Science and Technology of Education Department of Heilongjiang Province (No. 12531633)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Acupuncture Treatment Research Studies
- Health Sciences › Medicine › Neurology › Intracerebral and Subarachnoid Hemorrhage Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Keywords
- acupuncture, intracerebral hemorrhage, miR-23a-3p, ferroptosis, neuronal cell death, neuroinflammation, heme oxygenase-1, GPX4, cerebral edema, autologous blood transfusion, neuroprotection
- MeSH terms
- Ferroptosis, Animals, Cells, Cultured, Cerebral Hemorrhage, Male, Neurons, Down-Regulation, Acupuncture Therapy, Cytokines, Rats, Sprague-Dawley, Apoptosis, MicroRNAs, NF-E2-Related Factor 2, Rats
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