Precision-cut liver slices: a versatile tool to advance liver research
Open accessAbstract
Human precision-cut liver slices represent a robust and versatile ex vivo model which retains the complex and multi-cellular histoarchitecture of the hepatic environment. As such, they represent an ideal model to investigate the mechanisms of liver injury and for the identification of novel therapeutic targets. Schematic overview to highlight the utility of precision-cut liver slices as a relevant and versatile ex-vivo model of liver disease. Top panel; Precision cut liver slices (PCLS) exposed to ethanol develop mega-mitochondria, a classical hallmark of Alcoholic Liver Disease (ALD). Right panel; PCLS from liver tumours can be used as a model for liver cancer and can be used to investigate cancer-immune cell interactions by co-culturing with matched immune cells. Bottom panel; Exposure to a mixture of oleic and linoleic acids can simulate Non-Alcoholic Fatty Liver Disease (NAFLD). Left panel; PCLS can be infected with Hepatitis B and C virus and used as a model to study viral infection and replication.
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Induction of MASLD in vitro using FFAs leads to pronounced and quantitatively measurable lipid accumulation in cell lines within 24-72 h, contrasting with animal models where HFD induces steatosis in 8-16 weeks, steatohepatitis in 16-36 weeks, and fibrosis/cirrhosis in 24-52 weeks or more.
“Induction of MASLD in vitro using free fatty acids leads to a pronounced and quantitatively measurable accumulation of lipids in cell lines within 24–72 h [29].”
From The HepG2 Cell Line as a Model for Studying Metabolic Dysfunction-Associated Steatotic Liver Disease (2026) · cites this paper as [60]
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References 15 · Cited by 125
Research works printed but not linked: 12
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- Authors
- Palma, E., Doornebal, E. J., & Chokshi, S.
- Affiliations
- King's College London (GB)
- Corresponding author
- Chokshi, Shilpa
- Venue
- Hepatology International
- Published
- 2018-12-04
- Volume
- 13
- Issue
- 1
- Pages
- 51-57
- ISSN
- 1936-0533 (ISSN-L)
- 1936-0541
- PMC
- PMC6513823
- OpenAlex
- W2902912681
- Cited by
- 125
- References
- 15
- Citations by year
- 2026: 15 · 2025: 15 · 2024: 19 · 2023: 15 · 2022: 17 · 2021: 20 · 2020: 19 · 2019: 5
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Hepatology International (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Foundation for Liver Research
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- precision-cut liver slices, ex vivo liver model, liver injury mechanisms, alcoholic liver disease, liver tumors, liver cancer, non-alcoholic fatty liver disease, hepatitis B virus, hepatitis C virus
- MeSH terms
- Humans, Liver, Liver Diseases, Models, Biological, Organ Culture Techniques, Specimen Handling, Tumor Cells, Cultured
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