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Cited by 4Heavy Metal Pollution RemediationChromium effects and bioremediationAdsorption and biosorption for pollutant removal

Co-existence of Metal Transporter Genes and Heavy Metal Bioremediation Potential of Bacterial Strains from the Effluent Water

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Kaur, Harpreet, Katyal, Priya, Natt, Simranpreet Kaur, Vikal, Yogesh, Chandel, Sumita·2025·Indian Journal of Microbiology
DOI 10.1007/s12088-024-01362-yPMC PMC12579039
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Abstract

Heavy metal contamination and accumulation pose a major threat to all life forms in the environment due to its toxic nature, non-biodegradable properties and accumulative behavior. The present study aims to investigate the metals resistance mechanisms, removal capacity and bioremediation potential of the high metal-resistant indigenous bacterial species Bacillus thuringiensis strain BHR1, Bacillus cereus strain BHR2, Enterobacter cloacae strain BHR4, Bacillus pumilus strain BHR5, Bacillus altitudinis strain BHR6 and Klebsiella pneumonia strain BHR8 isolated from the effluent water. Moreover, the presence of specific metal transporter genes such as chrA, chrB, pbrA and nccA in these isolates indicated their role in metal resistance and removal. Based on minimum inhibitory concentration, bacterial isolates namely, BHR1 and BHR5 exhibited high metal tolerance against Cr (510 and 550 ppm), Pb (250 and 300 ppm), Ni (300 and 150 ppm) and As (200 and 120 ppm), respectively. The bioremediation potential of these bacterial isolates (BHR1 and BHR5) was evaluated under batch-scale conditions. Our results revealed that the addition of nutrients, as well as modification of pH to 6.0, enhances the bioremediation potential of BHR1 enabling the reduction of Pb, Cr, Ni and Zn by 60.9%, 31%, 22.7% and 22%, respectively. These findings suggested the potential application of these metal resistant bacterial isolates for the bioremediation of heavy metal contaminated effluents. Supplementary information The online version contains supplementary material available at 10.1007/s12088-024-01362-y.

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Authors
Kaur, H., Katyal, P., Natt, S. K., Vikal, Y., & Chandel, S.
Affiliations
Punjab Agricultural University (IN)
Corresponding author
Kaur, Harpreet
ORCID
Venue
Indian Journal of Microbiology
Publisher
Springer Science and Business Media LLC
Published
2024-08-02
Volume
65
Issue
3
Pages
1586-1601
ISSN
  • 0046-8991 (ISSN-L)
  • 0973-7715
DOI
10.1007/s12088-024-01362-y
PMC
PMC12579039
OpenAlex
W4401243472
Cited by
4
References
44
Citations by year
2026: 2 · 2025: 2
FWCI
0.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Indian Journal of Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion)
Funders
punjab pollution control board (4809)
Topics
  • Physical Sciences › Environmental Science › Water Science and Technology › Heavy Metal Pollution Remediation
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Chromium effects and bioremediation
  • Physical Sciences › Environmental Science › Water Science and Technology › Adsorption and biosorption for pollutant removal
Keywords
heavy metal bioremediation, metal resistance mechanisms, effluent, Bacillus thuringiensis, Bacillus pumilus, Enterobacter cloacae, Klebsiella pneumoniae, chromate resistance, lead removal, nickel removal, arsenic removal, zinc removal, minimum inhibitory concentration, industrial wastewater

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