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Cited by 8Identification and Quantification in FoodSpectroscopy and Chemometric AnalysesMeat and Animal Product Quality

High resolution melting is a useful tool to detect animal species sources of various milk types

Open access
Bulut·2023·Journal of Food Science and Technology
DOI 10.1007/s13197-023-05705-3PMC PMC10076476
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Abstract

Accurate identification of animal species sources in milk have become quite important due to adulteration of high-priced milk types in the dairy industry. To date, milk identification methods have mainly depended on biochemical properties or physical properties detected by spectroscopic methods. The current study aimed to develop an easy to use and sensitive DNA-based High resolution melting (HRM) assay to identify animal species and detect cross-adulteration of water buffalo, bovine, goat, sheep, camel and donkey milks. HRM compatible designed primer set, targeted mitochondrial region, successfully amplified the specific targeted region for six animal species DNA and showed a high degree of specificity based on nucleotide variations. Capillary electrophoresis analysis validated the specific amplicons and determined the amplicon lengths as 114 bp for bovine, goat, sheep, and camel, 115 bp for water buffalo, and 121 bp for donkey. HRM analysis showed a clear discrimination for water buffalo-bovine, camel-bovine and donkey-bovine adulteration down to 0.5%, and goat-sheep adulteration down to 1% in the milk admixtures. The efficacy of the method was also confirmed by its standard curve with a very high correlation coefficient In conclusion, the designed HRM assay allows for the rapid, sensitive and cost-effective authentication of milk and dairy products. Supplementary information The online version contains supplementary material available at 10.1007/s13197-023-05705-3.

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Sorted by citations

Authors
Bulut
Venue
Journal of Food Science and Technology
Publisher
Springer Science and Business Media LLC
Published
2023-02-28
Volume
60
Issue
5
Pages
1612-1620
ISSN
  • 0022-1155 (ISSN-L)
  • 0975-8402
DOI
10.1007/s13197-023-05705-3
PMC
PMC10076476
OpenAlex
W4322626551
Cited by
8
References
0
Citations by year
2026: 4 · 2024: 3 · 2023: 1
FWCI
0.71
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Food Science and Technology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
  • Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Meat and Animal Product Quality
Keywords
high-resolution melting analysis, milk adulteration, animal species identification, milk authentication, mitochondrial DNA, buffalo milk, cow's milk, goat milk, sheep milk, camel milk, donkey milk, dairy products

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