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Cited by 24Microbial Natural Products and BiosynthesisMicrobial Metabolism and ApplicationsBiopolymer Synthesis and Applications

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Optimization of iturin A production from Bacillus subtilis ZK-H2 in submerge fermentation by response surface methodology

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Yue, Hua, Zhong, Juan, Li, Zhemin, Zhou, Jinyan, Yang, Jie, Wei, Hongfei, Shu, Dan, Luo, Di, Tan, Hong·2021·3 Biotech
DOI 10.1007/s13205-020-02540-7PMC PMC7788149
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Abstract

In this research, single-factor and response surface experiments were conducted in the fed-batch fermentation process to improve the yield of iturin A. The effect of adding various concentrations of precursor amino acids l-asparagine (Asn), l-aspartic acid (Asp), l-glutamic acid (Glu), l-glutamine (Gln), l-Serine (Ser) and l-proline (Pro) at different adding times (3 and 12 h) on iturin A production and cell growth was studied. The respective addition of amino acids (Asp 0.28 g/L; Asn 0.36 g/L; Glu 0.20, 0.28 and 0.360 g/L; Gln 0.20, 0.28 and 0.36 g/L; Pro 0.12, 0.20, 0.28 and 0.36 g/L) at 3 h was shown to improve cell growth but did not affect the yield of iturin A. Meanwhile, the individual addition of the same amino acids at 12 h improved cell growth and increased the yield of iturin A. Excellent correlation was obtained between the predicted and measured values, suggesting that the regression model was accurate and reliable; highly significant (P < 0.0001), and the determination coefficient (R 2 = 0.975). When 0.0752 g/L Asn; 0.1992 g/L Gln and 0.1464 g/L Pro were added at 12 h, the yield of iturin A reached 0.85 g/L, which is 32.81%-fold higher than that of the initial process. Therefore, this study obtained optimal parameters for iturin A production by the experimental method, and process validation gave high iturin A yields (0.85 g/L) during a 60 h fermentation. These findings could guide an up-scaling of the fermentation process. Electronic supplementary material The online version of this article (10.1007/s13205-020-02540-7) contains supplementary material, which is available to authorized users.

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Metadata

Authors
Yue, H., Zhong, J., Li, Z., Zhou, J., Yang, J., Wei, H., Shu, D., Luo, D., & Tan, H.
Affiliations
  • Chinese Academy of Sciences (CN)
  • Zaozhuang University (CN)
Corresponding author
  • Zhong, Juan
  • Tan, Hong
ORCID
Venue
3 Biotech
Publisher
Springer Science and Business Media LLC
Published
2021-01-07
Volume
11
Issue
2
Pages
36-36
ISSN
  • 2190-572X (ISSN-L)
  • 2190-5738
DOI
10.1007/s13205-020-02540-7
PMC
PMC7788149
OpenAlex
W3118516953
Cited by
24
References
0
Citations by year
2025: 3 · 2024: 9 · 2023: 6 · 2022: 4 · 2021: 2
FWCI
3.06
Type
journal article
Language
en
Open access
Licence
Locations
  • 3 Biotech (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • the Chinese Academy of Sciences (CAS) Light of West China Program (2017XBZG_XBQNXZ_B_006)
  • the Key Laboratory of Environmental and Applied Microbiology of the Chengdu Institute of Biology CAS (KLCAS-2018-3)
  • Innovation Academy for Seed Design, CAS
Topics
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
Keywords
iturin A, Bacillus subtilis, fed-batch fermentation, amino acid precursors, asparagine, glutamine, proline, fermentation optimization, mycotoxin production, cell growth

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CoChat. (2026). Citation check: Optimization of iturin A production from Bacillus subtilis ZK-H2 in submerge fermentation by response surface methodology. https://app.cochat.ai/papers/10.1007/s13205-020-02540-7
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Yue, H., Zhong, J., Li, Z., Zhou, J., Yang, J., Wei, H., Shu, D., Luo, D., & Tan, H. (2021). Optimization of iturin A production from Bacillus subtilis ZK-H2 in submerge fermentation by response surface methodology. 3 Biotech. https://doi.org/10.1007/s13205-020-02540-7