The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Chemical composition, antibacterial and antioxidant activities of Piper betle and Anethum graveolens essential oils against Methicillin-resistant Staphylococcus aureus clinical isolates
Open accessAbstract
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infection worldwide due to its antimicrobial resistance. Plant-derived essential oils (EOs) have undergone extensive observational and clinical research to explore their antimicrobial properties. The present study aimed to check mec A positive MRSA isolates using sequencing analysis, determination of chemical composition using gas chromatography-mass spectroscopy (GC-MS), antioxidant, and antimicrobial activity of Anethum graveolens and Piper betle EOs against the infectious agent MRSA. The result demonstrates a significant antibacterial activity of both essential oils against MRSA clinical isolates. GC-MS analysis of Piper betle showed (41.06%) 3-Allyl-6-methoxyphenol (Eugenol) as the major compound, whereas Anethum graveolens exhibited o-cymene (14.01%) abundantly. Piper betle essential oil retained appreciable levels of total phenolic (39.5 ± 10.9 mg/g of gallic acid equivalents) and flavonoid content (216 ± 145 mg quercetin equivalent/g), when compared to Anethum graveolens essential oil. A strong correlation was observed between antioxidant activity (DPPH, FRAP, and ABTS), total phenolic, and total flavonoid content in the Piper betle and displayed using principal component analysis (PCA) and a scatter matrix plot. Parallelly, clear morphological bacterial alterations were visualized by scanning electron microscopy after treating it with essential oils. MRSA showed malformed cell surfaces or broken cells with pore formation and septae. These findings imply that both essential oils are potential natural sources of antimicrobials against the MRSA superbug. They can also be used in combination therapies with other plant EOs or with traditional antibiotics to combat the rise of bacteria resistance.
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Cited by
Raikwar, G., Mohan, S., & Dahiya, P. (2025) · Scientific Reports · doi:10.1038/s41598-025-18680-w · Cited by 3
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Metadata
- Authors
- Raikwar, G., Mohan, S., & Dahiya, P.
- Affiliations
- Amity University (IN)
- Corresponding author
- Mohan, Sumedha
- Dahiya, Praveen
- Venue
- Brazilian Journal of Microbiology
- Published
- 2025-01-30
- Volume
- 56
- Issue
- 2
- Pages
- 1017-1031
- ISSN
- 1517-8382 (ISSN-L)
- 1678-4405
- PMC
- PMC12095719
- OpenAlex
- W4406990552
- Cited by
- 9
- References
- 34
- Citations by year
- 2026: 8 · 2025: 1
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Brazilian Journal of Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion)
- Funders
- Council of Science and Technology, U.P. (CST/D-2298)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Piperaceae Chemical and Biological Studies
- Keywords
- methicillin-resistant Staphylococcus aureus, Piper betle, dill, essential oils, antibacterial activity, antioxidant activity, chemical composition, mecA gene, o-cymene, eugenol, total phenolic content, total flavonoid content, bacterial morphology, antimicrobial resistance, natural antimicrobials
- MeSH terms
- Anti-Bacterial Agents, Antioxidants, Humans, Gas Chromatography-Mass Spectrometry, Microbial Sensitivity Tests, Oils, Volatile, Plant Oils, Staphylococcal Infections, Anethum graveolens, Piper betle, Methicillin-Resistant Staphylococcus aureus
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