The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The assay was conducted according to Bradford [15].”
From Functional and Bioactive Characterization of Hemp Cake Proteins and Polyphenols from Non-Psychoactive Cannabis sativa (2025) · cites this paper as Bradford [15]
In the citing paper
“The Bradford method for protein determination (Bradford, 1976) is based on the colour change in Coomassie blue G250 when it reacts with proteins.”
From Chemical methods for the characterization of proteolysis in cheese during ripening (1997) · cites this paper as Bradford, 1976
Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION
Gornall, A. G., Bardawill, C. J., & David, M. M. (1949) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)57021-6 · Cited by 16,164
A rapid, sensitive, and specific method for the determination of protein in dilute solution
Schaffner, W., & Weissmann, C. (1973) · Analytical Biochemistry · doi:10.1016/0003-2697(73)90217-0 · Cited by 2,527
Reisner, A. H., Nemes, P., & Bucholtz, C. A. (1975) · Analytical Biochemistry · doi:10.1016/0003-2697(75)90461-3 · Cited by 618
Handbook of Biochemistry. Selected Data for Molecular Biology.
(1969) · Annals of Internal Medicine · doi:10.7326/0003-4819-70-1-239 · Cited by 559
PURIFICATION AND PROPERTIES OF FRUCTOSE-1,6-DIPHOSPHATASE
Mokrasch, L. C., & McGilvery, R. W. (1956) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)65205-6 · Cited by 282
Phenolic Hydroxyl Ionization in Proteins. I. Bovine Serum Albumin1-3
Tanford, C., & Roberts, G. L. (1952) · Journal of the American Chemical Society · doi:10.1021/ja01130a017 · Cited by 189
Ein einfaches Verfahren zur Analyse der Serumproteine und anderer Proteingemische
Graßmann, W., & HANNIG, K. (1950) · Die Naturwissenschaften · doi:10.1007/bf00623155 · Cited by 175
Determination of proteins in “Tris” buffer by the biuret reaction
Robson, R., Goll, D. E., & Temple, M. J. (1968) · Analytical Biochemistry · doi:10.1016/0003-2697(68)90188-7 · Cited by 145
Simple Microassay of Protein with Membrane Filter
KUNO, H., & Kihara, H. K. (1967) · Nature · doi:10.1038/215974a0 · Cited by 121
Research works printed but not linked: 4
- Kaul, 1970, J. Food Sci. Technol, 7, 11
- Swaminathan, 1973, Indian J. Exp. Biol, 11, 63
- Sherbon, 1974, J. Assoc. Off. Anal. Chem, 57, 1338
- Wetlaufer, 1962, Advan. Prot. Chem, 17, 378
Cited by
Sorted by citations
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Jefferson, R., Kavanagh, T. A., & Bevan, M. W. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb02730.x · Cited by 9,854
New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening
Skehan, P., Storeng, R., Scudiero, D. A., Monks, A. P., McMahon, J., Vistica, D. T., Warren, J. T., Bokesch, H. R., Kenney, S., & Boyd, M. R. (1990) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/82.13.1107 · Cited by 9,842
Pushko, P., Pumpens, P., & Grens, E. (2013) · Intervirology · doi:10.1159/000346773 · Cited by 5,975
Oxidative stress and some antioxidant systems in acid rain-treated bean plants
Velikova, V, Yordanov, I., & Edreva, A. (2000) · Plant Science · doi:10.1016/s0168-9452(99)00197-1 · Cited by 5,299
Calculation of protein extinction coefficients from amino acid sequence data.
Gill, S., & von Hippel, P. H. (1989) · Analytical Biochemistry · doi:10.1016/0003-2697(89)90602-7 · Cited by 5,382
Metadata
- Authors
- Bradford, M. M.
- Affiliations
- University of Georgia (US)
- Corresponding author
- Bradford, Marion M.
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 1976-05
- Volume
- 72
- Issue
- 1-2
- Pages
- 248-254
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W2128635872
- Cited by
- 255,302
- References
- 20
- Citations by year
- 2026: 4653 · 2025: 6079 · 2024: 6445 · 2023: 6652 · 2022: 7284 · 2021: 7677 · 2020: 7719 · 2019: 7373 · 2018: 7128 · 2017: 6899 · 2016: 7250 · 2015: 7450 · 2014: 7796 · 2013: 7638 · 2012: 7600
- FWCI
- 124.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- protein quantification, Coomassie Brilliant Blue G-250, dye-protein binding, colorimetric assay, absorption maximum shift, detergent interference, sodium dodecyl sulfate, Triton X-100
- MeSH terms
- Binding Sites, Colorimetry, Methods, Microchemistry, Protein Binding, Proteins, Rosaniline Dyes, Time Factors
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