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The determination of ϵ-amino groups in soluble and poorly soluble proteinaceous materials by a spectrophotometrie method using trinitrobenzenesulfonic acid
Abstract
A procedure using 2,4,6-trinitrobenzenesulfonic acid (TNBS) for the determination of epsilon-amino groups in soluble and poorly soluble proteinaceous materials is presented. The major modification from previous procedures is an extended TNBS reaction time to allow a stoichiometric reaction with amino groups. In addition, autoclave hydrolysis is used to assure sample dissolution for spectrophotometric measurements. The assay accuracy was evaluated by determining epsilon-amino groups of insulin and bovine albumin. The determinations differed from literature values by < or = 3.3%. The epsilon-amino group content of Type B gelatin was found to be 33.0 mol/gelatin molecule of 1000 residues and is in agreement with similar source gelatins and collagen. The coefficient of variation for determinations on all three materials was < or = 5.3%. The assay should be applicable to a broad range of proteinaceous materials.
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“For SF (Figure 1C), the main characteristic bands were observed at 1641 cm⁻¹, corresponding to C=O stretching (Amide I); 1514 cm⁻¹, associated with Amide II vibrations, including in-plane N-H bending and C-N stretching; and 1234 cm⁻¹, corresponding to Amide III, resulting from N-C stretching and C=O bending, related to β-sheet formation [27,36].”
Citations
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Metadata
- Authors
- Bubnis, W. A., & Ofner, C. M.
- Affiliations
- University of the Sciences (US)
- ORCID
- Ofner, Clyde M.
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 1992-11
- Volume
- 207
- Issue
- 1
- Pages
- 129-133
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W1970226578
- Cited by
- 265
- References
- 19
- Citations by year
- 2026: 3 · 2025: 10 · 2024: 7 · 2023: 8 · 2022: 4 · 2021: 12 · 2020: 6 · 2019: 11 · 2018: 13 · 2017: 12 · 2016: 15 · 2015: 19 · 2014: 13 · 2013: 19 · 2012: 20
- FWCI
- 0.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- epsilon-amino groups, 2,4,6-trinitrobenzene sulfonic acid, gelatin, collagen, insulin, bovine serum albumin, spectrophotometric assay
- MeSH terms
- Albumins, Amines, Animals, Cattle, Gelatin, Humans, Insulin, Molecular Weight, Proteins, Sensitivity and Specificity, Solubility, Spectrophotometry, Time Factors, Trinitrobenzenesulfonic Acid
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