The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
The oxidation of tiron by superoxide anion. Kinetics of the reaction in aqueous solution and in chloroplasts
Abstract
The rate of reaction between superoxide anion (O2) and 1,2-dihydroxybenzene-3,5-disulfonic acid (tiron) was measured with pulse radiolysis-generated O2. A kinetic spectrophotometric method utilizing competition between p-benzoquinone and tiron for O2 was employed. In this system, the known rate of reduction of p-benzoquinone was compared with the rate of oxidation of tiron to the semiquinone. From the concentration dependence of the rate of tiron oxidation, the absolute second order rate constant for the reaction was determined to be 5x10-8 M-minus1-s-minus1. Ascorbate reduced O2 to hydrogen peroxide with a rate constant of 10-8 M-minus1-s-minus1 as determined by the same method. The tiron semiquinone may be used as an indicator free radical for the formation of superoxide anion in biological systems because of the rapid rate of oxidation of the catechol by O2 compared to the rate of O2 formation is most enzymatic systems. Tiron oxidation was used to follow the formation of superoxide anion in swollen chloroplasts. The chloroplasts photochemically reduced molecular oxygen which was further reduced to hydrogen peroxide by tiron. Tiron oxidation specifically required O2 since O2 was consumed in the reaction and tiron did not reduce the P700 cation radical or other components of Photosystem I under anaerobic conditions.
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“Thus, in some assays, Oz reduces tetranitro methane [11], cytochrome c or nitro tetrazolium blue [100], and in others it oxidises epinephrine, tiron pyrogallol or 6-hydroxydopamine [101,102], Figure 38.”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [102]
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Cites
Acid dissociation constant and decay kinetics of the perhydroxyl radical
Béhar, D., Czapski, G., Rabani, J., Dorfman, L. M., & Schwarz, H. A. (1970) · The Journal of Physical Chemistry · doi:10.1021/j100711a009 · Cited by 499
Epel, B. L., & Neumann, J. (1973) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(73)90211-9 · Cited by 133
Superoxide reduction as a mechanism of ascorbate-stimulated oxygen uptake by isolated chloroplasts
Allen, J. F., & Hall, D. O. (1973) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(73)91016-4 · Cited by 109
Pulse radiolysis studies of electron transfer in aqueous quinone solutions
WILLSON, R. L. (1971) · Transactions of the Faraday Society · doi:10.1039/tf9716703020 · Cited by 102
Soluble & membrane-bound superoxide dismutases in a blue-green alga (spirulina) and spinach
Lumsden, J., & Hall, D. O. (1974) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(74)90887-0 · Cited by 75
The Tiron free radical as a sensitive indicator of chloroplastic photoautoxidation
Miller, R. W., & Macdowall, F. D. H. (1975) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(75)90062-6 · Cited by 69
Self-citationRapp, U., Adams, W. C., & Miller, R. W. (1973) · Canadian Journal of Biochemistry · doi:10.1139/o73-021 · Cited by 54
Self-citationSimic, M. G., & Hayon, E. (1973) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(73)90849-8 · Cited by 53
The Oxidation of Catechols by Reduced Flavins and Dehydrogenases
Miller, R. W., & Rapp, U. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43512-6 · Cited by 45
Self-citationDesign considerations for nanosecond pulse radiolysis studies using kinetic spectrophotometry
HUNT, J. W., Greenstock, C. L., & Bronskill, M. J. (1972) · International Journal for Radiation Physics and Chemistry · doi:10.1016/0020-7055(72)90011-3 · Cited by 40
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Research works printed but not linked: 1
- Adams, 1968, Vol. 1, 289
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Superoxide radical and superoxide dismutases
Fridovich, I. (1995) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.64.070195.000525 · Cited by 3,455
Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity
Andrés, C. M. C., Pérez de la Lastra, J. M., Andrés Juan, C., Plou, F. J., & Pérez-Lebeña, E. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24031841 · Cited by 342
Manganese, superoxide dismutase, and oxygen tolerance in some lactic acid bacteria
Archibald, F. S., & Fridovich, I. (1981) · Journal of Bacteriology · doi:10.1128/jb.146.3.928-936.1981 · Cited by 319
Rodrı́guez, E. M., Márquez, G., Tena, M., Álvarez, P. M., & Beltrán, F. J. (2014) · Applied Catalysis B: Environmental · doi:10.1016/j.apcatb.2014.11.002 · Cited by 274
NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells
Brar, S. S., Corbin, Z. A., Kennedy, T. P., Hemendinger, R. A., Thornton, L., Bommarius, B., Arnold, R. S., Whorton, A. R., Sturrock, A., Huecksteadt, T. P., et al. (2003) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00525.2002 · Cited by 262
Metadata
- Authors
- Greenstock, C. L., & Miller, R. W.
- Affiliations
- Atomic Energy (Canada) (CA)
- ORCID
- Miller, R.W.
- Venue
- Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Publisher
- Elsevier BV
- Published
- 1975-07
- Volume
- 396
- Issue
- 1
- Pages
- 11-16
- ISSN
- 0005-2728 (ISSN-L)
- OpenAlex
- W2075937164
- Cited by
- 157
- References
- 12
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 5 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 3 · 2017: 4 · 2016: 1 · 2015: 5 · 2014: 7 · 2013: 4 · 2012: 7
- FWCI
- 1.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochimica et Biophysica Acta (BBA) - Bioenergetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- Tiron, superoxide, p-benzoquinone, vitamin C, hydrogen peroxide, chloroplasts, photosystem I, superoxide detection, pulse radiolysis, rate constants
- MeSH terms
- Benzenesulfonates, Chloroplasts, Kinetics, Mathematics, Oxidation-Reduction, Oxygen, Plants, Superoxides, Time Factors, 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt, Triticum
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