Immunocytochemical localization of glycerol-3-phosphate dehydrogenase in rat oligodendrocytes
Abstract
In this study, two indirect immunoperoxidase staining procedures were used to investigate the cellular localization of rat brain glycerol-3-phospate dehydrogenase (EC 1.1.1.8;GPDH). At the light and electron microscopic level, we found that the use of monospecific rabbit antibodies to GPDH consistently resulted in the specific staining of only one glial cell population. GPDH-positive cells in perineuronal, interfascicular and perivascular positions were identified as oligodendrocytes by classical morphological criteria. The specificity of GPDH antigen-antibody reaction was determined by qualitative and quantitative immunochemical methods and by imunocytochemical controls for immunologic and methodologic sources of nonspecific reaction product. The illustrative data from this study serve to qualitatively define GPDH as a biochemical marker for oligodendrocytes in rat central nervous tissue. In view of the fact that the synthesis of rat brain GPDH is specifically regulated by glucocorticoids, the positive results obtained in this study further warrant the interpretation that rat oligodendrocytes are target cells for glucocorticoids.
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Oligodendrocytes are reportedly the only cells in the brain that express cytosolic glycerol-3-phosphate-dehydrogenase (GPD1).
“we further note that oligodendrocytes are reportedly the only cells in the brain that express cytosolic glycerol-3-phosphate-dehydrogenase (GPD1) (Cheng et al., 1997;Leveille et al., 1980;Nguyen et al., 2003).”
From Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons (2024) · cites this paper as Leveille et al., 1980
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- Authors
- Leveille, P. J., McGinnis, J. F., Maxwell, D. S., & De Vellis, J.
- Affiliations
- University of California, Los Angeles (US)
- ORCID
- Maxwell, David S.
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1980-09
- Volume
- 196
- Issue
- 2
- Pages
- 287-305
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2047292511
- Cited by
- 135
- References
- 0
- Citations by year
- 2024: 2 · 2022: 1 · 2021: 1 · 2013: 2 · 2012: 2
- FWCI
- 4.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- glycerol-3-phosphate dehydrogenase, oligodendrocytes, immunocytochemical localization, oligodendrocyte markers, glucocorticoid regulation, rat central nervous system, glial cells, perivascular cells
- MeSH terms
- Animals, Cerebellum, Glucocorticoids, Glycerolphosphate Dehydrogenase, Immunoenzyme Techniques, Microscopy, Electron, Neuroglia, Oligodendroglia, Rats
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