Stimulation of phosphoinositide hydrolysis by trans-(±)-ACPD is greatly enhanced when astrocytes are cultured in a serum-free defined medium
Abstract
Recent studies have demonstrated that astrocytes have much greater abilities to produce and respond to signalling molecules in the CNS than had been previously estimated. We now report a dramatic enhancement in the ability of a glutamate metabotropic receptor agonist, 1-aminocyclopentane-trans-(+/-)-1,3-dicarboxylic acid (trans-(+/-)-ACPD, to stimulate phosphoinositide hydrolysis in astrocytes cultured in a serum-free defined medium compared with astrocytes cultured in conventional serum-containing medium (43.2 +/- 3.6 vs. 3.2 +/- 0.48-fold of basal, respectively). This enhancement was selective to trans-(+/-)-ACPD as little or no difference in the response to carbachol or norepinephrine was seen between the two culture conditions. These results indicate a great potential for the phosphoinositide pathway in astrocyte glutamatergic signal transduction.
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Astrocytes take up glutamate through Na+-dependent glutamate transporters, Cl−-dependent transport mechanisms, and Ca2+-dependent transport systems.
“Studies show that astrocytes can take up Glu through Na+-dependent Glu transporters, Cl--dependent transport mechanisms, and Ca²+-dependent transport systems (174).”
From Nociplastic pain: the prominent role of non-neuronal cells in central and peripheral sensitization (2026) · cites this paper as [174]
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Nociplastic pain: the prominent role of non-neuronal cells in central and peripheral sensitization
Lai, W., Tang, X., Chen, S., Zhou, Y., Qi, Z., Cao, M., Yuan, C., Yu, J., & Wen, C. (2026) · Frontiers in Immunology · doi:10.3389/fimmu.2026.1677310 · Cited by 4 · see citing works
- Authors
- Miller, S., Bridges, R. J., & Cotman, C. W.
- Affiliations
- University of California, Irvine (US)
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1993-07
- Volume
- 618
- Issue
- 1
- Pages
- 175-178
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2092777123
- Cited by
- 19
- References
- 0
- Citations by year
- 2026: 1
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- astrocytes, phosphoinositide hydrolysis, trans-ACPD, metabotropic glutamate receptors, glutamate signalling, serum-free defined medium, phosphoinositide pathway, carbachol, norepinephrine
- MeSH terms
- Animals, Astrocytes, Cells, Cultured, Cycloleucine, Hydrolysis, Phosphatidylinositols, Culture Media, Serum-Free, Receptors, Metabotropic Glutamate, Rats
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