Relationship between resting cytosolic Ca2+ and responses induced byN-methyl-d-aspartate in hippocampal neurons
Abstract
Cytosolic calcium concentrations ([Ca2+]i) in cultured hippocampal neurons from rat embryos were measured using fura-2. Neurons with higher resting [Ca2+]i showed greater [Ca2+]i responses to N-methyl-D-aspartate (NMDA) and K+ depolarization. There was a strong relationship between resting [Ca2+]i and the maximal changes in [Ca2+]i (delta[Ca2+]i), which fit the our proposed equation to describe this relationship.
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The resting level of intracellular Ca2+ is 20-50 nM.
“relative to the resting level of intracellular Ca2+ (20–50 nM) (109–111)”
From Synaptic memory and CaMKII (2023) · cites this paper as [109]
The unusually high Hill coefficient of ∼6 for CaMKII and the high requirement for Ca2+ compared to resting Ca2+ ensures maintenance of synapse specificity and the Hebbian nature of LTP.
“The unusually high Hill coefficient of ∼6 for CaMKII (108) and the high requirement for Ca2+ (half maximal of ∼1.6 µM at saturating CaM) compared to the resting level of Ca2+ (20–50 nM) (109, 110) ensures maintenance of the synapse specificity and Hebbian nature of LTP (19).”
From Synaptic memory and CaMKII (2023) · cites this paper as [109]
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Estimating Intracellular Calcium Concentrations and Buffering without Wavelength Ratioing
Maravall, M., Mainen, Z. F., Sabatini, B. L., & Svoboda, K. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76809-3 · Cited by 416 · see citing works
Nicoll, R. A., & Schulman, H. (2023) · Physiological Reviews · doi:10.1152/physrev.00034.2022 · Cited by 126 · see citing works
“…when CaM is not limiting (apparent K d ¼ 1.6 l M for autophosphorylation) (108) relative to the resting level of intracellular Ca 2 1 (20 – 50 nM) (109 – 111) and the unusually high Hill coef fi cient of (cid:4) 6 for CaMKII (108) ensure maintenance of the synapse speci fi city and Hebbian nature…”
BackgroundInfluential
- Authors
- Nakajima, K., Harada, K., Ebina, Y., Yoshimura, T., Ito, H., Ban, T., & Shingai, R.
- Affiliations
- Yamaguchi University (JP)
- Iwate University (JP)
- Corresponding author
- Nakajima, Kazuki
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1993-02
- Volume
- 603
- Issue
- 2
- Pages
- 321-323
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W1968468332
- Cited by
- 18
- References
- 0
- Citations by year
- 2025: 3 · 2023: 2
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (03750358)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Keywords
- NMDA receptor, hippocampal neurons, calcium response, potassium depolarization, fura-2, calcium homeostasis
- MeSH terms
- Animals, Calcium, Cells, Cultured, Cytosol, Embryo, Mammalian, Hippocampus, Kainic Acid, Kinetics, Membrane Potentials, Microscopy, Fluorescence, Neurons, Potassium Chloride, N-Methylaspartate, Fura-2, Rats
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