The DOI matches its Crossref record; 38 of the 39 references it cites were found in a registry or an index.
The distribution of 5-HT1A and 5-HT2A receptor mRNA in human brain
Abstract
We have examined the distribution of 5-HT1A and 5-HT2A receptor mRNAs in post-mortem human hippocampus, neocortex, raphe nuclei, cerebellum and basal ganglia using in situ hybridization histochemistry. Receptor transcripts in brains from two males and two females (mean age +/- S.D. = 70 +/- 4 years; post-mortem interval = 29 +/- 6 h) were visualised with 35S-radiolabelled synthetic oligodeoxyribonucleic acid probes. In the hippocampus, 5-HT1A receptor mRNA was present in all fields, especially CA1. In the parahippocampal gyrus and neocortical regions 5-HT1A receptor mRNA was enhanced in superficial and middle laminae. 5-HT1A receptor mRNA was particularly abundant in the raphe and other serotonergic cell groups of the brainstem. The analysis of emulsion dipped sections showed 5-HT1A receptor mRNA to be concentrated in pyramidal neurons, together with the granule cells of the dentate gyrus. In neocortical areas lamina III pyramidal neurons were more heavily labelled than those in lamina V. There was no evidence of glial expression of 5-HT1A receptor mRNA in grey matter or white matter compartments. 5-HT2A receptor mRNA was present in all neocortical areas examined, where it was located in pyramidal neurons, of lamina V more than in those of lamina III, as well as in putative interneurons, especially within lamina IVc of the striate cortex. 5-HT2A receptor mRNA was observed at minimal levels in the hippocampus and not in the raphe. Neither 5-HT1A nor 5-HT2A receptor mRNA were detected in the cerebellum, substantia nigra or striatum. The ability to detect these transcripts at the regional and cellular level will help reveal important details of the 5-HT receptor system in the human brain. This includes the investigation of their putative roles in the normal chemoarchitecture and in pathophysiological brain processes.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“A similar pattern of 5-HT2A receptor expression has been shown in human post-mortem tissue using both autoradiography (211) and in situ hybridization (212).”
From Psychedelics and Other Psychoplastogens for Treating Mental Illness. (2021) · cites this paper as [212]
Citations
Cites
THE BASIC UNIFORMITY IN STRUCTURE OF THE NEOCORTEX
Röckel, A., Hiorns, R. W., & Powell, T. P. S. (1980) · Brain · doi:10.1093/brain/103.2.221 · Cited by 890
Pompeiano, M., Palacios, J. M., & Mengod, G. (1994) · Molecular Brain Research · doi:10.1016/0169-328x(94)90223-2 · Cited by 870
Pompeiano, M., Palacios, J., & Mengod, G. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-02-00440.1992 · Cited by 699
Serotonin receptors in the human brain—III. Autoradiographic mapping of serotonin-1 receptors
Pazos, Á., Probst, A., & Palacios, J. M. (1987) · Neuroscience · doi:10.1016/0306-4522(87)90326-5 · Cited by 684
Zifa, E., & Fillion, G. (2001) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(01)80026-2 · Cited by 677
Molecular biology of 5-HT receptors
Boess, F., & Martin, I. L. (1994) · Neuropharmacology · doi:10.1016/0028-3908(94)90059-0 · Cited by 675
Araneda, R. C., & Andrade, R. (1991) · Neuroscience · doi:10.1016/0306-4522(91)90128-b · Cited by 621
Peroutka, S. J. (1993) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1993.tb03166.x · Cited by 524
Kobilka, B. K., Frielle, T., Collins, S., Yang-Feng, T. L., Kobilka, T. S., Francke, U., Lefkowitz, R. J., & Caron, M. G. (1987) · Nature · doi:10.1038/329075a0 · Cited by 515
Chalmers, D. T., & Watson, S. J. (1991) · Brain Research · doi:10.1016/0006-8993(91)90748-k · Cited by 388
Serotonin receptors in the human brain—IV. Autoradiographic mapping of serotonin-2 receptors
Pazos, A., Probst, A., & Palacios, J. M. (1987) · Neuroscience · doi:10.1016/0306-4522(87)90327-7 · Cited by 355
Serotonin Uptake Sites and Serotonin Receptors Are Altered in the Limbic System of Schizophrenics
Joyce, J. N., Shane, A. L., Lexow, N., Winokur, A., Casanova, M. F., & Kleinman, J. E. (1993) · Neuropsychopharmacology · doi:10.1038/npp.1993.32 · Cited by 340
OʼKusky, J. R., & Colonnier, M. (1982) · The Journal of Comparative Neurology · doi:10.1002/cne.902100307 · Cited by 335
Hrdina, P. D., Demeter, E., Vu, T. B., Sótonyi, P., & Palkovits, M. (1993) · Brain Research · doi:10.1016/0006-8993(93)91015-k · Cited by 327
Pre‐and Postmortem Influences on Brain RNA
BARTON, A. J. L., Pearson, R. C. A., Najlerahim, A., & Harrison, P. J. (1993) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1993.tb03532.x · Cited by 269
Self-citationSelective Abnormalities of Prefrontal Serotonergic Receptors in Schizophrenia
Laruelle, M. A. (1993) · Archives of General Psychiatry · doi:10.1001/archpsyc.1993.01820220066007 · Cited by 235
Morilak, D. A., Garlow, S. J., & Ciaranello, R. D. (1993) · Neuroscience · doi:10.1016/0306-4522(93)90241-7 · Cited by 231
Francis, P. T., Sims, N. R., PROCTER, A. W., & Bowen, D. M. (1993) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1993.tb13381.x · Cited by 231
Dillon, K. A., Gross‐Isseroff, R., Israeli, M., & Biegon, A. R. (1991) · Brain Research · doi:10.1016/0006-8993(91)90171-q · Cited by 198
Serotonin Receptor Changes in Dementia of the Alzheimer Type
Cross, A. J., Crow, T. J., Ferrier, I. N., Johnson, J. A., Bloom, S. R., & Corsellis, J. (1984) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1984.tb06081.x · Cited by 195
Whitaker‐Azmitia, P. M., Clarke, C., & Azmitia, E. C. (1993) · Synapse · doi:10.1002/syn.890140303 · Cited by 181
Cloning of the human serotonin 5-HT2 and 5-HT1C receptor subtypes
Saltzman, A. G., Morse, B., Whitman, M. M., Ivanshchenko, Y., Jaye, M., & Felder, S. (1991) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(91)92105-s · Cited by 168
Brain 5-HT1 binding sites in depressed suicides
Cheetham, S. C., Crompton, M. R., Katona, C., & Horton, R. W. (1990) · Psychopharmacology · doi:10.1007/bf02247138 · Cited by 165
Whitaker‐Azmitia, P. M., & Azmitia, E. C. (1989) · Brain Research · doi:10.1016/0006-8993(89)90972-4 · Cited by 141
Ashby, C. R., Edwards, E., & Wang, R. Y. (1994) · Synapse · doi:10.1002/syn.890170306 · Cited by 116
Lowther, S., Paermentier, F. D., Crompton, M. R., Katona, C., & Horton, R. W. (1994) · Brain Research · doi:10.1016/0006-8993(94)90932-6 · Cited by 97
Burnet, P. W. J., Michelson, D., Smith, M. A., Gold, P. W., & Sternberg, E. M. (1994) · Brain Research · doi:10.1016/0006-8993(94)90664-5 · Cited by 94
Self-citationCytoarchitecture of serotonin‐synthesizing neurons in the pontine tegmentum of the human brain
Baker, K. G., Halliday, G. M., Halasz, P., Hornung, J., Geffen, L. B., Cotton, R. G. H., & Törk, I. (1991) · Synapse · doi:10.1002/syn.890070407 · Cited by 90
Expression of Serotonin Transporter Messenger RNA in the Human Brain
Austin, M. C., Bradley, C. C., Mann, J. J., & Blakely, R. (1994) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1994.62062362.x · Cited by 85
Gellman, R. L., & Aghajanian, G. K. (1994) · Neuroscience · doi:10.1016/0306-4522(94)90077-9 · Cited by 80
Francis, P. T., Pangalos, M. N., Pearson, R. C. A., Middlemiss, D. N., Stratmann, G. C., & Bowen, D. M. (1992) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)11149-x · Cited by 78
Morilak, D. A., Somogyi, P., Luján, R., & Ciaranello, R. D. (1994) · Neuropsychopharmacology · doi:10.1038/sj.npp.1380102 · Cited by 68
Burnet, P. W. J., Eastwood, S. L., & Harrison, P. J. (1994) · Neuroscience Letters · doi:10.1016/0304-3940(94)90296-8 · Cited by 62
Self-citationGérard, C., Langlois, X., Gingrich, J. A., Doucet, E., Vergé, D., Kia, H. K., Raisman‐Vozari, R., GOZLAN, H., Mestikawy, S. E., & Hamon, M. (1994) · Neuroscience · doi:10.1016/0306-4522(94)90472-3 · Cited by 42
Localization and gene expression of serotonin1A (5HT1A) receptors in human brain postmortem
Marazziti, D., Marracci, S., Palego, L., Rotondò, A., Mazzanti, C. M., Nardi, I., Ladinsky, H., Giraldo, E., Borsini, F., & Cassano, G. B. (1994) · Brain Research · doi:10.1016/s0006-8993(09)90010-5 · Cited by 37
Neuronal localization of 5-HTIA receptor mRNA and protein in rat embryonic brain stem cultures
Hillion, J., Catelon, J., Riad, M., Hamon, M., & Vitry, F. D. (1994) · Developmental Brain Research · doi:10.1016/0165-3806(94)90124-4 · Cited by 37
Ding, D., Tóth, M., Zhou, Y., Parks, C. L., Hoffman, B. J., & Shenk, T. E. (1993) · Molecular Brain Research · doi:10.1016/0169-328x(93)90040-v · Cited by 37
Production and characterization of a specific 5-HT2 receptor antibody
Garlow, S. J., Morilak, D. A., Dean, R. R., Roth, B. L., & Ciaranello, R. D. (1993) · Brain Research · doi:10.1016/0006-8993(93)91121-8 · Cited by 35
Research works printed but not linked: 5
- Austin, 1994, Localization of 5-HT2A receptor mRNA in neurons of the human brain stem, Soc. Neurosci. Abstr., 20, 1546
- Hirst, 1994, Expression of 5-HT1A, 5-HT2A and 5-HT2C receptors in astrocytes, Soc. Neurosci. Abstr., 20, 1547
- Lopez, 1994, Distribution of 5-HT1A receptor mRNA in the human hippocampus, Soc. Neurosci. Abstr., 20, 1547
- Rahman, S., McLean, J.H., Darby-King, A., Paterno, G., Reynolds, J.N. and Neuman, R.S., Loss of cortical serotonin 5-HT2A signal transduction in senescent rats: reversal following inhibition of protein kinase C, Neuroscience, in press.
- Shapiro, 1994, Distribution of the serotonin1A receptor serotonin uptake site and tryptophan hydroxylase-like immunoreactivity (TpH-Li) in the human dorsal raphe, Soc. Neurosci. Abstr., 20, 1547
Cited by
Sorted by citations
A review of central 5-HT receptors and their function
Barnes, N. M., & Sharp, T. (1999) · Neuropharmacology · doi:10.1016/s0028-3908(99)00010-6 · Cited by 3,211
Nichols, D. E. (2016) · Pharmacological Reviews · doi:10.1124/pr.115.011478 · Cited by 1,762
The neuropathology of schizophrenia
Harrison, P. J. (1999) · Brain · doi:10.1093/brain/122.4.593 · Cited by 1,677
Self-citationNichols, D. E. (2004) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2003.11.002 · Cited by 1,211
Nichols, D. E., & Nichols, C. D. (2008) · Chemical Reviews · doi:10.1021/cr078224o · Cited by 895
Metadata
- Authors
- Burnet, P. W. J., Eastwood, S. L., Lacey, K., & Harrison, P. J.
- Affiliations
- Warneford Hospital (GB)
- Corresponding author
- Burnet, P.W.J.
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1995-04
- Volume
- 676
- Issue
- 1
- Pages
- 157-168
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2058711144
- Cited by
- 283
- References
- 39
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 6 · 2023: 4 · 2022: 3 · 2021: 7 · 2020: 5 · 2019: 11 · 2018: 6 · 2017: 4 · 2016: 6 · 2015: 5 · 2014: 9 · 2013: 5 · 2012: 7
- FWCI
- 4.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- 5-HT1A receptor, 5-HT2A receptor, serotonin receptors, receptor mRNA distribution, human brain, hippocampus, neocortex, raphe nuclei, pyramidal neurons, laminae, dentate gyrus granule cells, postmortem brain
- MeSH terms
- Aged, Autoradiography, Basal Ganglia, Brain, Brain Stem, Cerebellum, Cerebral Cortex, Female, Hippocampus, Humans, Male, Receptors, Serotonin, RNA, Messenger, Tissue Distribution, In Situ Hybridization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
