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A solubilizable acrylamide gel for electrophoresis
Open accessAbstract
N,N'-Diallyl-tartardiamide (DATD) if used mole for mole in place of methylene-bis acrylamide in the preparation of acrylamide gels [l] results in a product which dissolves in 2% periodic acid in 20-30 min at room temperature or in ca. 10 min at 37'.The properties of the DATD gels are very similar to ordinary gels; they are not tacky, are readily sliced and stain in the usual fashion.The gels take somewhat longer to set, 1 hr being required for the frequently used 7% gel; they do not suffer if kept at room temperature overnight.In order to insure good solution of alkaline gels it is necessary to neutralize these with acetic acid before immersion in the periodic acid.Proteins are generally not attacked by periodic acid and can be recovered by ap propriate procedures after dissolution of the gels.Perio-
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Cited by
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Heine, J. W., Honess, R. W., Cassai, E., & Roizman, B. (1974) · Journal of Virology · doi:10.1128/jvi.14.3.640-651.1974 · Cited by 503
Fritz, J. D., Swartz, D. R., & Greaser, M. L. (1989) · Analytical Biochemistry · doi:10.1016/0003-2697(89)90116-4 · Cited by 254
Metadata
- Authors
- Anker, H. S.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1970-04-16
- Volume
- 7
- Issue
- 3
- Pages
- 293-293
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2049972861
- Cited by
- 274
- References
- 0
- FWCI
- 7.76
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Anker, H. S. (1970). A solubilizable acrylamide gel for electrophoresis. FEBS Letters. https://doi.org/10.1016/0014-5793(70)80185-5
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