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Sequence‐dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H
Open accessAbstract
We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo‐ and 2′‐O‐methylnucleosides, a nonaribonucleotide, [5′‐32 pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3′m(UG)d(AATG)m(GAC)5′ was used as a hybridization strand, *pACUUACCUG was cleaved between C6 and C7 to yield *pACUUAC. In the presence of 3′m(UGAA)d(TGGA)m(C)5′, the nonaribonucleotide was hydrolyzed, mainly between U8 and C9, to give *pACUUACCU. This method will have a variety of applications in the field of RNA engineering.
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Cited by
Structure of ribonuclease H phased at 2 a resolution by MAD analysis of the selenomethionyl protein
Yang, W., Hendrickson, W. A., Crouch, R. J., & Satow, Y. (1990) · Science · doi:10.1126/science.2169648 · Cited by 473
How does RNase H recognize a DNA.RNA hybrid?
Nakamura, H., Oda, Y., Iwai, S., Inoue, H., Ohtsuka, E., Kanaya, S., Kimura, S., Katsuda, C., Katayanagi, K., & Morikawa, K. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.24.11535 · Cited by 193
Self-citationKanaya, S., Kohara, A., Miura, Y., Sekiguchi, A., Iwai, S., Inoue, H., Ohtsuka, E., & Ikehara, M. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39607-3 · Cited by 180
Self-citationEgli, M., Usman, N., & Rich, A. (1993) · Biochemistry · doi:10.1021/bi00064a004 · Cited by 145
Kanaya, S., Katsuda-Nakai, C., & Ikehara, M. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99002-2 · Cited by 116
Metadata
- Authors
- Inoue, H., Hayase, Y., Iwai, S., & Ohtsuka, E.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1987-05-11
- Volume
- 215
- Issue
- 2
- Pages
- 327-330
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2010299178
- Cited by
- 263
- References
- 0
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Inoue, H., Hayase, Y., Iwai, S., & Ohtsuka, E. (1987). Sequence‐dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H. FEBS Letters. https://doi.org/10.1016/0014-5793(87)80171-0
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