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Complete amino acid sequence of human placental 17β-hydroxysteroid dehydrogenase deduced from cDNA
Abstract
cDNA clones for 17 beta-hydroxysteroid dehydrogenase (17-HSD; EC 1.1.1.62) were isolated from a placental lambda gt11 expression library using polyclonal antibodies against placental 17-HSD. The largest cDNA contained 1325 nucleotides, consisting of a short 5'-noncoding segment, a coding segment of 987 nucleotides terminated by a TAA codon, and a 329 nucleotide long 3'-noncoding segment. The open reading frame encoded a polypeptide of 327 amino acid residues with a predicted Mr of 34853. The amino acid sequence of 23 N-terminal amino acids determined from purified 17-HSD agreed with the sequence deduced from cDNA. The deduced amino acid sequence also contained two peptides previously characterized from the proposed catalytic area of placental 17-HSD.
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“In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD (503-505).”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [503]
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Metadata
- Authors
- Peltoketo, H., Isomaa, V., Mäentausta, O., & Vihko, R.
- Affiliations
- University of Oulu (FI)
- Corresponding author
- Peltoketo, H.
- ORCID
- Peltoketo, H.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1988-10-24
- Volume
- 239
- Issue
- 1
- Pages
- 73-77
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2073005394
- Cited by
- 294
- References
- 26
- Citations by year
- 2025: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 4 · 2013: 1 · 2012: 3
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- 17β-hydroxysteroid dehydrogenase, placenta, cDNA cloning, amino acid sequence, open reading frames, N-terminal sequence, steroid metabolism
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Female, Humans, Molecular Sequence Data, Placenta, Pregnancy, Protein Conformation, 17-Hydroxysteroid Dehydrogenases, Restriction Mapping
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