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Cited by 82Immune Cell Function and InteractionImmunotherapy and Immune ResponsesImmune cells in cancer

The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.

Large scale production of human lymphokine activated killer cells for use in adoptive immunotherapy

Mesler Muul, Linda, Director, Elaine P., Hyatt, Cornelia L., Rosenberg, Steven A.·1986·Journal of Immunological Methods
DOI 10.1016/0022-1759(86)90015-3
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Abstract

Immunotherapy utilizing the adoptive transfer of lymphokine activated killer (LAK) cells in conjunction with recombinant interleukin 2 (RIL-2) is capable of reducing established metastatic cancer in a variety of animal tumor models. A major difficulty in the application of these efforts to the treatment of human cancer has been the activation in vitro of up to 2 X 10(11) human peripheral blood lymphocytes obtained by repeated leukaphereses. We have thus developed optimal and simplified techniques for the generation of human LAK cells for use in clinical trials. We have found that 1.5 X 10(9) lymphocytes separated on Ficoll-Hypaque gradients and incubated in 1000 ml of culture medium in a 2.3 liter roller bottle with 1000-1500 U of RIL-2 per ml, generated LAK cells capable of killing fresh human tumor cells in a 4 h chromium release assay. The culture medium used was RPMI 1640 with 2 mM glutamine, 2% heat-inactivated human AB serum, 50 micrograms/ml streptomycin and gentamicin and 50 U/ml penicillin. This technique allows activation of sufficient numbers of cells in a research laboratory setting to conduct human clinical trials. The administration of LAK cells generated in this fashion can mediate the regression of human tumors when administered in conjunction with IL-2.

Overview

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Crossref · 10.1016/0022-1759(86)90015-3

Cites

14 of 14 references verified0 citation errors · 0 not checkable

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Citations

Cites

Show all 14 references

Research works printed but not linked: 5

  1. Chang, 1984, J. Biol. Response Mod., 3, 561
  2. Grimm, 1984, Lymphokines, 9, 279
  3. Lafreniere, 1985, Cancer Res., 45, 3735
  4. Lotze, 1981, Cancer Res., 41, 4420
  5. Mazumder, 1982, Cancer Res., 42, 913

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Metadata

Authors
Mesler Muul, L., Director, E. P., Hyatt, C. L., & Rosenberg, S. A.
Affiliations
National Institutes of Health (US)
ORCID
Rosenberg, Steven A.
Venue
Journal of Immunological Methods
Publisher
Elsevier BV
Published
1986-04
Volume
88
Issue
2
Pages
265-275
ISSN
0022-1759 (ISSN-L)
DOI
10.1016/0022-1759(86)90015-3
OpenAlex
W2015192166
Cited by
82
References
14
Citations by year
2020: 1 · 2019: 1 · 2014: 1
FWCI
5.16
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Immunological Methods (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Cell Function and Interaction
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
Keywords
lymphokine-activated killer cells, adoptive immunotherapy, interleukin-2, large-scale cell production, peripheral blood lymphocytes, leukapheresis, roller bottle culture, tumor regression, metastatic cancer, clinical trials
MeSH terms
Animals, Cell Separation, Cells, Cultured, Culture Media, Humans, Immunotherapy, Interleukin-2, Killer Cells, Natural, Kinetics, Neoplasms, T-Lymphocytes, Cytotoxic, Mice

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