Streptomycin-induced, third-position misreading of the genetic code
Abstract
Streptomycin was used to increase the frequency of errors in protein synthesis in vivo. In the system under study two misreading errors were observed. Both involved the erroneous insertion of lysine at asparagine codons, because of misreading of a pyrimidine as a purine at the 3' position of the codon. Streptomycin increased the errors at the two codons AAU and AAC to the same extent, thereby maintaining the error ratio found for basal level mistranslation.
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AAC and AAU differ in misreading rate by tRNALys, leading to Asn replaced by Lys.
“However, AAC and AAU also differ in misreading rate, in particular by tRNALys, which ideally should decode only AAA and AAG codons but does misread AAC and AAU, leading to Asn replaced by Lys.”
From Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines (2021) · cites this paper as [39]
The misreading error rate is six times greater for AAU than for AAC, with the error ratio consistently maintained under Asn-starved and non-starved conditions and with Streptomycin.
“This misreading error rate is six times greater for AAU than for AAC, with the error ratio consistently maintained in different experimental settings, e.g., under both Asn-starved and non-starved conditions [38], or with Streptomycin used to inhibit translation [39].”
From Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines (2021) · cites this paper as [39]
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Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines
Xia, X. (2021) · Vaccines · doi:10.3390/vaccines9070734 · Cited by 201 · see citing works
- Authors
- Johnston, T. C., & Parker, J.
- Affiliations
- Southern Illinois University Carbondale (US)
- Corresponding author
- Parker, Jack
- ORCID
- Johnston, Timothy C.
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1985-01
- Volume
- 181
- Issue
- 2
- Pages
- 313-315
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W1968129586
- Cited by
- 28
- References
- 0
- Citations by year
- 2023: 1 · 2021: 1 · 2020: 3 · 2018: 6 · 2017: 1
- FWCI
- 0.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- streptomycin, codon misreading, genetic code, translation errors, protein synthesis errors, AAC
- MeSH terms
- Autoradiography, Capsid, Electrophoresis, Polyacrylamide Gel, Genetic Code, Isoelectric Focusing, Lysine, Streptomycin, RNA-Binding Proteins, Capsid Proteins
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