Validation and use of centrifugal ultrafiltration-dialysis in the measurement of percent free oestradiol in serum
Abstract
The validity of the centrifugal ultrafiltration-dialysis method in the measurement of the percentage of oestradiol and testosterone which is not bound to protein (free) in human serum was assessed. In an initial validation, the values of % free steroid obtained for oestradiol and testosterone in protein-free ultrafiltrates of human serum were higher than expected. Filter paper discs used in the system were shown to adsorb [3H]oestradiol from protein-free solution. Their replacement with glass-fibre discs, which did not adsorb oestradiol, decreased but did not eliminate the discrepancy between observed and expected results for % free oestradiol in ultrafiltrates. The use of polyethylene rather than polypropylene vials then reduced the discrepancy to insignificant levels. The estimates for % free oestradiol in serum were reduced by these modifications.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
UF has technical pitfalls: non-specific binding, steroid adsorption to the filtration membrane, and protein leakage through the filtration membrane.
“Most common pitfalls of UF are due to non-specific binding, steroid adsorption to the filtration membrane and protein leakage through the filtration membrane [200, 203].”
From Role of sex hormone-binding globulin in the free hormone hypothesis and the relevance of free testosterone in androgen physiology (2022) · cites this paper as [203]
Cited by
Sorted by citations
Shaw, R. W. (1992) · Fertility and Sterility · doi:10.1016/s0015-0282(16)55205-4 · Cited by 119 · see citing works
Narinx, N., David, K., Walravens, J., Vermeersch, P., Claessens, F., Fiers, T., Lapauw, B., Antonio, L., & Vanderschueren, D. (2022) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-022-04562-1 · Cited by 96 · see citing works
- Authors
- Dowsett, M., Mansfield, M. D., Griggs, D. J., & Jeffcoate, S. L.
- Corresponding author
- Dowsett, M.
- Venue
- Journal of Steroid Biochemistry
- Publisher
- Elsevier BV
- Published
- 1984-09
- Volume
- 21
- Issue
- 3
- Pages
- 343-345
- ISSN
- 0022-4731 (ISSN-L)
- OpenAlex
- W2015678678
- Cited by
- 28
- References
- 0
- Citations by year
- 2022: 1 · 2014: 1
- FWCI
- 2.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Steroid Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Keywords
- free testosterone, assay validation
- MeSH terms
- Centrifugation, Density Gradient, Dialysis, Estradiol, Female, Humans, Testosterone, Ultrafiltration
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
