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The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
Open accessAbstract
The ability of purified U1 small nuclear RNA-protein complexes (U1 snRNPs) to bind in vitro to two RNAs transcribed from recombinant DNA clones by bacteriophage T7 RNA polymerase has been studied. A transcript which contains sequences corresponding to the small intron and flanking exons of the major mouse beta-globin gene is bound in marked preference to an RNA devoid of splice site sequences. The site of U1 snRNP binding to the globin RNA has been defined by T1 ribonuclease digestion of the RNA-U1 snRNP complex. A 15-17-nucleotide region, including the 5' splice site, remains undigested and complexed with the snRNP such that it can be co-precipitated by antibodies directed against the U1 snRNP. Partial proteinase K digestion of the U1 snRNP abolishes interaction with the globin RNA, indicating that the snRNP proteins contribute significantly to RNA binding. No RNA cleavage, splicing, or recognition of the 3' splice site by U1 snRNPs has been detected. Our results are discussed in terms of the probable role of U1 snRNPs in the messenger RNA splicing of eucaryotic cell nuclei.
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Cited by
Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., & Green, M. R. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.18.7035 · Cited by 6,324
Protein–protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
Kohtz, J. D., Jamison, S. F., Will, C. L., Zuo, P., Lührmann, R., Garcia-Blanco, M. A., & Manley, J. L. (1994) · Nature · doi:10.1038/368119a0 · Cited by 623
Query, C. C., Bentley, R. C., & Keene, J. D. (1989) · Cell · doi:10.1016/0092-8674(89)90175-x · Cited by 580
Izant, J. G., & Weintraub, H. (1984) · Cell · doi:10.1016/0092-8674(84)90050-3 · Cited by 549
Structure of spliceosomal snRNPs and their role in pre-mRNA splicing
Lührmann, R., Kastner, B., & Bach, M. (1990) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(90)90001-i · Cited by 403
Metadata
- Authors
- Mount, S. M., Pettersson, I., Hinterberger, M., Karmas, A., & Steitz, J. A.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1983-06
- Volume
- 33
- Issue
- 2
- Pages
- 509-518
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1995478437
- Cited by
- 565
- References
- 0
- FWCI
- 27.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Mount, S. M., Pettersson, I., Hinterberger, M., Karmas, A., & Steitz, J. A. (1983). The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell. https://doi.org/10.1016/0092-8674(83)90432-4
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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