The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Semi-intact cells permeable to macromolecules: Use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex
Open accessAbstract
We introduce a new method that removes portions of the plasma membrane of eukaryotic cells to form semi-intact cells. During preparation, these cells lose their soluble cytoplasmic contents, but retain secretory organelles such as the ER and Golgi complex in an intact form. Transport of protein between the ER and Golgi can be functionally reconstituted in vitro using these semi-intact cells by incubation in the presence of cytosol and ATP. Export of the vesicular stomatitis virus strain tsO45 G protein from the ER in vitro is temperature-sensitive, similar to the result observed in vivo. These cells allow direct access of chemicals and antibodies to the cytoplasmic domain of the cell and may be a widely applicable model system for study of a broad range of problems in cell biology.
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Cites
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Self-citationSaraste, J., & Kuismanen, E. (1984) · Cell · doi:10.1016/0092-8674(84)90508-7 · Cited by 531
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Cited by
Molecular dissection of the secretory pathway
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Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
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Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum.
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Diffusional Mobility of Golgi Proteins in Membranes of Living Cells
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Metadata
- Authors
- Beckers, C. J. M., Keller, D. S., & Balch, W. E.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1987-08
- Volume
- 50
- Issue
- 4
- Pages
- 523-534
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2024739169
- Cited by
- 318
- References
- 41
- FWCI
- 11.20
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Beckers, C. J. M., Keller, D. S., & Balch, W. E. (1987). Semi-intact cells permeable to macromolecules: Use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex. Cell. https://doi.org/10.1016/0092-8674(87)90025-0
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
