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An in vitro system for the editing of apolipoprotein B mRNA

Driscoll, Donna M., Wynne, Judy K., Wallis, Simon C., Scott, James·1989·Cell
DOI 10.1016/0092-8674(89)90432-7
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Abstract

A novel form of RNA editing generates two forms of apolipoprotein B (apo-B) mRNA by converting C at nucleotide 6666 to U or a U-like base. We have established an in vitro system for the editing of apo-B mRNA using synthetic RNAs and S100 extracts from rat hepatoma cells. Editing was detected by a sensitive primer extension assay and confirmed by DNA sequencing. The in vitro editing activity is specific and sensitive to proteinase K. Apo-B100 RNAs were synthesized in vitro from deletion mutants spanning nucleotide 6666. Synthetic RNAs containing 2383, 483, and 55 nucleotides of apo-B mRNA sequence were edited in vitro with similar efficiency, but an RNA containing 26 nucleotides was not edited.

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Research works printed but not linked: 19

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Metadata

Authors
Driscoll, D. M., Wynne, J. K., Wallis, S. C., & Scott, J.
Venue
Cell
Publisher
Elsevier BV
Published
1989-08
Volume
58
Issue
3
Pages
519-525
ISSN
0092-8674
DOI
10.1016/0092-8674(89)90432-7
Cited by
132
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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