The DOI matches its Crossref record; 25 of the 26 references it cites were found in a registry or an index.
Mechanism of specificity in the Fos-Jun oncoprotein heterodimer
Abstract
Fos and Jun, the protein products of the nuclear proto-oncogenes c-fos and c-jun, associate preferentially to form a heterodimer that binds to DNA and modulates transcription of a wide variety of genes in response to mitogenic stimuli. Both Fos and Jun contain a single leucine zipper region. Previous studies have shown that the leucine zippers of Fos and Jun are necessary and sufficient to mediate preferential heterodimer formation. The leucine zipper regions from Fos and Jun are also known to fold autonomously, most likely as two-stranded, parallel coiled coils. We show here that 8 amino acids from Fos and from Jun are sufficient to mediate preferential heterodimer formation in a background of the GCN4 leucine zipper sequence. Using pH titration and amino acid replacements, we also show that destabilization of the Fos homodimer by acidic residues provides a major thermodynamic driving force for preferential heterodimer formation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Changing the amino acids at the e and g positions can be sufficient to change the dimerization specificity of a leucine zipper (Schuermann et al., 1991; O'Shea et al., 1992, 1993; Vinson et al., 1993; John et al., 1994; Zhou et al., 1994a, 1994b).”
From Oligomerization properties of GCN4 leucine zipper e and g position mutants (1997) · cites this paper as O'Shea et al., 1992
Citations
Cites
The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding Proteins
Landschulz, W. H., Johnson, P. F., & McKnight, S. L. (1988) · Science · doi:10.1126/science.3289117 · Cited by 3,443
Spectroscopic Determination of Tryptophan and Tyrosine in Proteins *
Edelhoch, H. (1967) · Biochemistry · doi:10.1021/bi00859a010 · Cited by 3,438
Spragg, S. P. (1979) · Nature · doi:10.1038/278085a0 · Cited by 1,707
Fos and jun: The AP-1 connection
Curran, T., & Franza, B. R. (1988) · Cell · doi:10.1016/0092-8674(88)90024-4 · Cited by 1,595
The packing of α-helices: simple coiled-coils
Crick, F. H. C. (1953) · Acta Crystallographica · doi:10.1107/s0365110x53001964 · Cited by 1,578
X-Ray Structure of the GCN4 Leucine Zipper, a Two-Stranded, Parallel Coiled Coil
O’Shea, E. K., Klemm, J. D., Kim, P. S., & Alber, T. (1991) · Science · doi:10.1126/science.1948029 · Cited by 1,444
Self-citationBohmann, D., Bos, T. J., Admon, A., Nishimura, T., Vogt, P. K., & Tjian, R. (1987) · Science · doi:10.1126/science.2825349 · Cited by 1,321
Evidence That the Leucine Zipper Is a Coiled Coil
O’Shea, E. K., Rutkowski, R., & Kim, P. S. (1989) · Science · doi:10.1126/science.2911757 · Cited by 837
Self-citationTropomyosin coiled-coil interactions: Evidence for an unstaggered structure
McLachlan, A., & Stewart, M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80119-7 · Cited by 664
Beveren, C. V., van Straaten, F., Curran, T., Müller, R., & Verma, I. M. (1983) · Cell · doi:10.1016/0092-8674(83)90306-9 · Cited by 524
Cited by
Sorted by citations
Moi, P., Chan, K., Asunis, I., Cao, A., & Kan, Y. W. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.21.9926 · Cited by 1,607
Reversible Hydrogels from Self-Assembling Artificial Proteins
Petka, W. A., Harden, J. L., McGrath, K. P., Wirtz, D., & Tirrell, D. A. (1998) · Science · doi:10.1126/science.281.5375.389 · Cited by 1,075
Coiled coils: a highly versatile protein folding motif.
Burkhard, P., Stetefeld, J., & Strelkov, S. (2001) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)01898-5 · Cited by 1,055
Protein-protein interactions: methods for detection and analysis
Phizicky, E. M., & Fields, S. (1995) · Microbiological Reviews · doi:10.1128/mr.59.1.94-123.1995 · Cited by 977
A spring-loaded mechanism for the conformational change of influenza hemagglutinin
Carr, C. M., & Kim, P. S. (1993) · Cell · doi:10.1016/0092-8674(93)90260-w · Cited by 857
Self-citation
Metadata
- Authors
- O'Shea, E. K., Rutkowski, R., & Kim, P. S.
- Affiliations
- Howard Hughes Medical Institute (US)
- Massachusetts Institute of Technology (US)
- ORCID
- Kim, Peter S.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1992-02
- Volume
- 68
- Issue
- 4
- Pages
- 699-708
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2066101080
- Cited by
- 422
- References
- 26
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 7 · 2021: 4 · 2020: 3 · 2019: 8 · 2018: 3 · 2017: 9 · 2016: 5 · 2015: 7 · 2014: 7 · 2013: 7 · 2012: 14
- FWCI
- 13.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Rita Allen Foundation
- Allen Foundation
- National Institutes of Health (2r01gm044162-10)
- National Institute of General Medical Sciences (GM44162)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
- Keywords
- Fos-Jun heterodimer, leucine zipper, c-fos, c-Jun, protein-protein interaction specificity, coiled coil, GCN4 leucine zipper, acidic residues, pH titration, amino acid substitutions, transcriptional regulation, mitogenic signaling
- MeSH terms
- Amino Acid Sequence, Hydrogen-Ion Concentration, Models, Molecular, Molecular Sequence Data, Protein Conformation, Thermodynamics, Molecular Structure, Leucine Zippers, Proto-Oncogene Proteins c-jun, Proto-Oncogene Proteins c-fos
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
