Identification and expression cloning of a leptin receptor, OB-R.
Open accessAbstract
The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [125I]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepared from mouse choroid plexus and screened with a leptin-AP fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Genetic mapping of the gene encoding OB-R shows that it is within the 5.1 cM interval of mouse chromosome 4 that contains the db locus.
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The leptin receptor was identified biochemically and shown to be a cytokine family receptor that is expressed broadly.
“The leptin receptor was identifiedbiochemically and shown tobe a cytokine family receptor that is expressed broadly (24).”
From Leptin at 14 y of age: an ongoing story (2009) · cites this paper as [24]
Leptin acts through the leptin receptor, a single-transmembrane-domain receptor of the cytokine receptor family.
“Leptin acts through the leptin receptor, a single-transmembrane-domain receptor of the cytokine receptor family.97”
From Pubertal development and regulation (2016) · cites this paper as [97]
Deficiency of leptin or insulin, or of their receptors in the CNS, results in hyperphagia, weight gain, and insulin resistance.
“deficiency of either hormone or their respective receptors in the CNS results in hyperphagia, weight gain and insulin resistance (27, 174, 183, 212).”
From Leptin and the Central Nervous System Control of Glucose Metabolism (2011) · cites this paper as [183]
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References 38 · Cited by 3,496
- Authors
- Tartaglia, L. A., Dembski, M., Weng, X., Deng, N., Culpepper, J., Devos, R., Richards, G. J., Campfield, L. A., Clark, F. T., Deeds, J., Muir, C., Sanker, S., Moriarty, A., Moore, K. J., Smutko, J. S., Mays, G. G., Wool, E. A., Monroe, C. A., & Tepper, R. I.
- Affiliations
- Takeda Oncology (United States) (US)
- F. Hoffmann-La Roche AG (CH)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 83
- Issue
- 7
- Pages
- 1263-1271
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2025846514
- Cited by
- 3,496
- References
- 38
- Citations by year
- 2026: 23 · 2025: 36 · 2024: 45 · 2023: 43 · 2022: 52 · 2021: 75 · 2020: 57 · 2019: 75 · 2018: 79 · 2017: 76 · 2016: 89 · 2015: 98 · 2014: 145 · 2013: 122 · 2012: 133
- FWCI
- 74.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- leptin receptor, leptin, choroid plexus, hypothalamus, IL-6 receptor, G-CSF receptor, LIF receptor, gene mapping, mouse chromosome 4, body weight regulation
- MeSH terms
- Amino Acid Sequence, Animals, Binding Sites, Choroid Plexus, Chromosome Mapping, Cloning, Molecular, Humans, Mice, Inbred C57BL, Mice, Obese, Molecular Sequence Data, Obesity, Proteins, Receptors, Cell Surface, RNA, Messenger, Gene Expression, Leptin, Mice, Receptors, Leptin
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