The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Kinetics and mechanism of heterogeneous hydrolysis of poly[(R)-3-hydroxybutyrate] film by PHA depolymerases
Abstract
The kinetics and mechanism of enzymatic degradation on the surface of poly[(R)-3-hydroxybutyrate] (P[(R)-3HB]) film have been studied using three types of extracellular poly(hydroxyalkanoate) (PHA) depolymerases from Alcaligenes faecalis, Pseudomonas picketti and Comamonas testosteroni. The monomer and dimer of 3-hydroxybutyric acid were produced during the course of the enzymatic degradation of P[(R)-3HB] film, and the rate of production was determined by monitoring the increase in absorbance at 210 nm on a spectrophotometer. The rate of enzymatic degradation increased to a maximum value with the concentration of PHA depolymerase, followed by a gradual decrease. The kinetic data were accounted for in terms of a heterogeneous enzymatic reaction, involving enzymatic degradation on the surface of P[(R)-3HB] film via two steps of adsorption and hydrolysis by a PHA depolymerase with binding and catalytic domains. The kinetic results suggest that the properties of the catalytic domains are very similar among the three PHA depolymerases, but that those of the binding domains are strongly dependent on the type of depolymerase.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Because of this mechanism of action, adding a higher amount of enzyme increases the efficiency of the degradation up to an inhibitory concentration that is detrimental to the degradation rates, owing to saturation of the substrate that prevents the catalytic site from interacting with the polymer.497”
From Catalysis as an Enabling Science for Sustainable Polymers (2018) · cites this paper as [497]
In the citing paper
“Depending on the provenance of the PHB depolymerase, either monomer and dimer of 3-hydroxybutyric acid 497 or oligomers can be obtained.[498][499][500]”
From Catalysis as an Enabling Science for Sustainable Polymers (2018) · cites this paper as [497]
Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates
Anderson, A. J., & DAWES, E. A. (1990) · Microbiological Reviews · doi:10.1128/mr.54.4.450-472.1990 · Cited by 2,917
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families
Gilkes, N. R., Henrissat, B., Kilburn, D. G., Miller, R. C., & Warren, R. A. J. (1991) · Microbiological Reviews · doi:10.1128/mr.55.2.303-315.1991 · Cited by 962
Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications
Wong, K. K. Y., Tan, L. U. L., & Saddler, J. N. (1988) · Microbiological Reviews · doi:10.1128/mr.52.3.305-317.1988 · Cited by 824
Doi, Y., Kanesawa, Y., Kunioka, M., & Saito, T. (1990) · Macromolecules · doi:10.1021/ma00203a006 · Cited by 515
Self-citationMultiplicity of beta-1,4-xylanase in microorganisms: functions and applications.
Wong, K. K. Y., Tan, L. U. L., & Saddler, J. N. (1988) · Microbiological Reviews · doi:10.1128/mmbr.52.3.305-317.1988 · Cited by 398
Doi, Y., Tamaki, A., Kunioka, M., & Soga, K. (1988) · Applied Microbiology and Biotechnology · doi:10.1007/bf00268190 · Cited by 342
Self-citationAn Extracellular Poly(3‐Hydroxybutyrate) Depolymerase from Alcaligenes faecalis
Tanio, T., Fukui, T., Shirakura, Y., Saito, T., Tomita, K., Kaiho, T., & Masamune, S. (1982) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1982.tb05907.x · Cited by 330
Decomposition of Poly-β-Hydroxybutyrate by Pseudomonads
Delafield, F. P., Doudoroff, M. J., Palleroni, N. J., Lusty, C. J., & Contopoulos, R. (1965) · Journal of Bacteriology · doi:10.1128/jb.90.5.1455-1466.1965 · Cited by 242
Watanabe, T., Suzuki, K., Oyanagi, W., Ohnishi, K., & Tanaka, H. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55449-1 · Cited by 227
Jendrossek, D., Knoke, I., Habibian, R. B., Steinb�chel, A., & Schlegel, H. G. (1993) · Journal of Polymers and the Environment · doi:10.1007/bf01457653 · Cited by 188
Shirakura, Y., Fukui, T., Saito, T., Okamoto, Y., Narikawa, T., Koide, K., Tomita, K., Takemasa, T., & Masamune, S. (1986) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(86)90118-2 · Cited by 187
Biodegradation of polyhydroxyalkanoates
Mergaert, J., Anderson, C. S., Wouters, A., Swings, J., & Kersters, K. (1992) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1992.tb05853.x · Cited by 186
Saito, T., Suzuki, K., Yamamoto, J., Fukui, T., Miwa, K., Tomita, K., Nakanishi, S., Odani, S., Suzuki, J., & Ishikawa, K. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.1.184-189.1989 · Cited by 174
Schirmer, A., Jendrossek, D., & Schlegel, H. G. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.4.1220-1227.1993 · Cited by 157
Poly-?-hydroxybutters�ure abbauende Bakterien und Exoenzym
Chowdhury, A. A. (1963) · Archives of Microbiology · doi:10.1007/bf00422523 · Cited by 149
Nakayama, K., Saito, T., Fukui, T., Shirakura, Y., & Tomita, K. (1985) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(85)90101-3 · Cited by 133
Enzymatic degradation of poly(hydroxyalkanoates) by a marine bacterium
Mukai, K., Yamada, K., & Doi, Y. (1993) · Polymer Degradation and Stability · doi:10.1016/0141-3910(93)90066-r · Cited by 133
Self-citationOccurrence of Poly-β-Hydroxybutyric Acid in Aerobic Gram-Negative Bacteria
Forsyth, W. G. C., HAYWARD, A. C., & ROBERTS, J. B. (1958) · Nature · doi:10.1038/182800a0 · Cited by 127
Brucato, C. L., & Wong, S. S. (1991) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(91)90572-z · Cited by 117
Cloning and high-level expression of chitinase-encoding gene of Streptomyces plicatus
Robbins, P. W., Overbye, K. M., Albright, C. F., Benfield, B., & Pero, J. G. (1992) · Gene · doi:10.1016/0378-1119(92)90604-n · Cited by 112
Kemnitzer, J. E., McCarthy, S. P., & Gross, R. A. (1992) · Macromolecules · doi:10.1021/ma00048a011 · Cited by 102
Enzymatic degradation of poly(hydroxyalkanoates) by Pseudomonas pickettii
Yamada, K., Mukai, K., & Doi, Y. (1993) · International Journal of Biological Macromolecules · doi:10.1016/0141-8130(93)90040-s · Cited by 98
Self-citationFukui, T., Narikawa, T., Miwa, K., Shirakura, Y., Saito, T., & Tomita, K. (1988) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(88)90112-4 · Cited by 97
Ilyobacter delafieldii sp. nov., a metabolically restricted anaerobic bacterium fermenting PHB
Janssen, P. H., & Harfoot, C. G. (1990) · Archives of Microbiology · doi:10.1007/bf00248964 · Cited by 91
Purification and properties of poly(3-hydroxyvaleric acid) depolymerase from Pseudomonas lemoignei
M�ller, B., & Jendrossek, D. (1993) · Applied Microbiology and Biotechnology · doi:10.1007/bf00242943 · Cited by 77
An extracellular d(−)-3-hydroxybutyrate oligomer hydrolase from Alcaligenes faecalis
Shirakura, Y., Fukui, T., Tanio, T., Nakayama, K., Matsuno, R., & Tomita, K. (1983) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(83)90310-2 · Cited by 68
Extracellular poly(hydroxyalkanoate) depolymerases and their inhibitor from Pseudomonas lemoignei
Mukai, K., Yamada, K., & Doi, Y. (1992) · International Journal of Biological Macromolecules · doi:10.1016/s0141-8130(05)80034-0 · Cited by 36
Self-citation
Cited by
Sorted by citations
Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters
Sudesh, K., Abe, H., & Doi, Y. (2000) · Progress in Polymer Science · doi:10.1016/s0079-6700(00)00035-6 · Cited by 2,262
Self-citationCatalysis as an Enabling Science for Sustainable Polymers
Zhang, X., Fevre, M., Jones, G. O., & Waymouth, R. M. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00329 · Cited by 936
Cutinase-Catalyzed Hydrolysis of Poly(ethylene terephthalate)
Ronkvist, Å. M., Xie, W., Lu, W., & Gross, R. A. (2009) · Macromolecules · doi:10.1021/ma9005318 · Cited by 710
Lifetime prediction of biodegradable polymers
Laycock, B. G., Nikolić, M., Colwell, J. M., Gauthier, E., Halley, P. J., Bottle, S. E., & George, G. A. (2017) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2017.02.004 · Cited by 678
Microbial degradation of polyurethane, polyester polyurethanes and polyether polyurethanes
Nakajima-Kambe, T., Shigeno-Akutsu, Y., Nomura, N., Onuma, F., & Nakahara, T. (1999) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051373 · Cited by 284
Metadata
- Authors
- Mukai, K., Yamada, K., & Doi, Y.
- Affiliations
- RIKEN (JP)
- Corresponding author
- Doi, Yoshiharu
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 1993-12
- Volume
- 15
- Issue
- 6
- Pages
- 361-366
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2019749929
- Cited by
- 143
- References
- 27
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 8 · 2023: 8 · 2022: 4 · 2021: 7 · 2020: 5 · 2019: 2 · 2018: 3 · 2017: 7 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 4.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Keywords
- poly[(R)-3-hydroxybutyrate], PHA depolymerase, enzyme kinetics, enzyme mechanism, Alcaligenes faecalis, Comamonas testosteroni, beta-hydroxybutyrate, enzyme adsorption, binding domain, catalytic domain, biodegradable plastics
- MeSH terms
- Polyhydroxybutyrates, Alcaligenes, Carboxylic Ester Hydrolases, Hydrolysis, Hydroxybutyrates, Kinetics, Polyesters, Pseudomonas, Solubility
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
