Intracellular injection of Lucifer yellow into cortical neurons in lightly fixed sections and its application to human autopsy material
Abstract
In this paper a method is described for injecting Lucifer yellow intracellularly in lightly fixed cortical slices. The resultant filling reveals detailed neuronal morphology of somata, dendrites, and their appendages offering the following advantages over currently available cell staining techniques: (1) a large number (10-30) of neurons can be filled intracellularly in a single experiment, (2) when coupled with retrograde fluorescent tracing methods, specific populations of neurons can be chosen for study, (3) good results can be obtained reliably, (4) neurons from autopsy or biopsy material can be intracellularly filled, (5) other neuronal features can be identified by combining this method with lectin or neurotransmitter immunocytochemistry, and (6) filled cells can be made electron dense, permitting specimens to be examined under the electron microscope.
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Intracellular injections of marker substances in fixed brain tissue is currently considered the best approach to visualize the dendritic spines in the human brain and is a well-established technique.
“Another method involving intracellular injections of marker substances in fixed brain tissue is currently considered the best approach to visualize the dendritic spines in the human brain and is a well-established technique (Einstein, 1988; Buhl et al., 1990).”
From The influence of phospho-tau on dendritic spines of cortical pyramidal neurons in patients with Alzheimer’s disease (2013) · cites this paper as Einstein, 1988
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Merino-Serrais, P., Benavides-Piccione, R., Blazquez-Llorca, L., Kastanauskaite, A., Rábano, A., Avila, J., & DeFelipe, J. (2013) · Brain · doi:10.1093/brain/awt088 · Cited by 134 · see citing works
- Authors
- Einstein, G.
- Affiliations
- Duke Medical Center (US)
- Corresponding author
- Einstein, Gillian
- ORCID
- Einstein, Gillian
- Venue
- Journal of Neuroscience Methods
- Publisher
- Elsevier BV
- Published
- 1988-12
- Volume
- 26
- Issue
- 2
- Pages
- 95-103
- ISSN
- 0165-0270 (ISSN-L)
- OpenAlex
- W1995961115
- Cited by
- 58
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2020: 1 · 2017: 1 · 2016: 1 · 2014: 1 · 2013: 1
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroscience Methods (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG05 128)
- National Eye Institute (EY-06661, EY05546)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Anatomy › Medical and Biological Sciences
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Keywords
- Lucifer Yellow, intracellular injection, neuronal morphology, cortical neurons, retrograde tracing, dendrites, soma
- MeSH terms
- Animals, Cats, Cerebral Cortex, Fluorescent Dyes, Humans, Isoquinolines, Tupaiidae
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