The patch-clamp technique in the study of secretion
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One of the basic cellular functions of virtually every cell type is the exocytotic release of molecules synthesized, stored and packaged into intracellular vesicles or granules. Over decades much effort has been concentrated on elucidating the chain of events leading to exocytosis. Unfortunately, the nature of the process that ultimately induces membrane fusion is not known, nor has it been established definitively whether or not the final steps in the secretory cascade are identical in different cells. Although the fusion between vesicle and plasma membrane has been neatly documented by electron micrographs, it was only recently that the technique of time-resolved membrane capacitance measurement has provided a more detailed insight into mechanistic aspects of exocytosis, both in terms of the fusion event and the steps involved in stimulus-secretion coupling.
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Capacitance measurements report the increase in cell surface area that occurs when the secretory granule fuses with the plasma membrane.
“Measurements of membrane capacitance report the increase in cell surface area that occurs when the secretory granule fuses with the plasma membrane (334, 505) (Figure 17A).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [505]
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Research works printed but not linked: 8
- Katz, B. (1969) The Release of Neural TransmitterSubstances Liverpool University Press
- Llinas, R., McGuiness, T. L., Leonard, C. S., Sugimori, M. and Greengard, P. (1985) Proc. Natl Acad. 5ci. USA 82, 3035-3039
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- Neher, E. and Penner, R. (1988) in/vlolecularMechanisms in Secretion (Thorn, N. A., Treiman, M. and Petersen, O. H., eds), pp. 262-270, Munksgaard 43
- Penner, R. (1988) Proc. NatlAcad. Sci. USA 85, 9856-9860
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Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
Role of Calcium in Angiotensin II-Mediated Aldosterone Secretion*
BARRETT, P. Q., BOLLAG, W. B., ISALES, C. M., MCCARTHY, R. T., & RASMUSSEN, H. (1989) · Endocrine Reviews · doi:10.1210/edrv-10-4-496 · Cited by 183 · see citing works
- Authors
- Penner, R., & Neher, E.
- Affiliations
- Max Planck Society (DE)
- Venue
- Trends in Neurosciences
- Publisher
- Elsevier BV
- Published
- 1989-01
- Volume
- 12
- Issue
- 4
- Pages
- 159-163
- ISSN
- 0166-2236 (ISSN-L)
- OpenAlex
- W1999703505
- Cited by
- 97
- References
- 0
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2020: 3 · 2017: 1 · 2016: 1 · 2012: 3
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Trends in Neurosciences (publishedVersion)
- PubMed (publishedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- exocytosis, membrane fusion, stimulus-secretion coupling, vesicle exocytosis, patch clamp, intracellular vesicles
- MeSH terms
- Adrenal Glands, Animals, Electrophysiology, Mast Cells, Membrane Potentials
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