The DOI matches its Crossref record; 52 of the 57 references it cites were found in a registry or an index.
1,25-Dihydroxyvitamin D3 regulates the synthesis of nerve growth factor in primary cultures of glial cells
Abstract
The effect of 1,25-dihydroxyvitamin D3 (1,25-(OH)2 D3) on nerve growth factor (NGF) synthesis was investigated in primary cultures of astrocytes prepared from brain of neonatal rats. 1,25-(OH)2 D3 elicited a dose-dependent increase of NGF mRNA with a maximal effect at 10(-7) M, which persisted for at least 48 h. Northern blot analysis revealed an expression of the vitamin D3 receptor (VDR) gene in primary glial cells. Treatment of cells with 1,25-(OH)2 D3 led to an increase in the VDR mRNA levels. Similar results were obtained in C6 glioma cells. Exposure of primary glial cells to 10(-8) M 1,25-(OH)2 D3 caused only a 2-fold increase of the levels of cell-secreted NGF after 3 days of treatment. However, a 5-fold increase was observed three days after a second addition of vitamin D3. Likewise, a pretreatment with lower doses of hormone such as 10(-10) M or 10(-9) M enhanced the responsiveness of the cells to a 24 h treatment with 10(-8) M hormone. It appears, therefore, that the duration of the treatment influences the level of synthesis of NGF, possibly as a consequence of the increase of the VDR gene expression. The specificity of 1,25-(OH)2 D3 is supported by the fact that a concentration of 10(-7) M of an another vitamin D3 metabolite, 24,25-(OH)2 D3, had no effect on NGF synthesis. Several lines of evidence indicate that astrocytes constitute the major cell type responsive to 1,25-(OH)2 D3 in primary cultures of glial cells.(ABSTRACT TRUNCATED AT 250 WORDS)
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“Vitamin D analogues have been shown to upregulate NGF in cultured glial cell and embryonic hippocampal cells [177,178], to decrease the percentage of cultured hippocampal cells undergoing mitosis [178], and to decrease BDNF and concommitantly to improve memory in postmenopausal women [179].”
From Vitamin D and Autism Spectrum Disorder: A Literature Review (2016) · cites this paper as [177]
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Cui, C., Cui, J., Jin, F., Cui, Y., Li, R., Jiang, X., Tian, Y., Wang, R. C., Jiang, P., & Gao, J. (2017) · Cellular Physiology and Biochemistry · doi:10.1159/000479571 · Cited by 5,214
Distribution of the vitamin D receptor and 1α-hydroxylase in human brain
Eyles, D. W., Smith, S., Kinobe, R., Hewison, M., & McGrath, J. J. (2005) · Journal of Chemical Neuroanatomy · doi:10.1016/j.jchemneu.2004.08.006 · Cited by 1,444
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New clues about vitamin D functions in the nervous system
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Metadata
- Authors
- Neveu, I., Naveilhan, P., Jehan, F., Baudet, C., Wion, D., De Luca, H. F., & Brachet, P.
- Affiliations
- Inserm (FR)
- Corresponding author
- Brachet, Philippe
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 1994-07
- Volume
- 24
- Issue
- 1-4
- Pages
- 70-76
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2072586872
- Cited by
- 241
- References
- 57
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 8 · 2023: 6 · 2022: 7 · 2021: 11 · 2020: 11 · 2019: 10 · 2018: 9 · 2017: 7 · 2016: 13 · 2015: 9 · 2014: 13 · 2013: 10 · 2012: 14
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Vitamin D Research Studies
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- 1,25-dihydroxyvitamin D3, vitamin D receptor, nerve growth factor, NGF synthesis, astrocytes, glial cells, NGF mRNA, C6 glioma cells, microglia, oligodendrocytes, meningeal fibroblasts, hormone responsiveness, treatment duration, 24,25-dihydroxyvitamin D3, neurotrophic factors, neonatal rat brain, vitamin D
- MeSH terms
- Animals, Animals, Newborn, Astrocytes, Brain, Calcitriol, Cells, Cultured, Dose-Response Relationship, Drug, Glioma, Kinetics, Nerve Growth Factors, RNA, Messenger, Time Factors, Tumor Cells, Cultured, Blotting, Northern, 24,25-Dihydroxyvitamin D 3, Gene Expression, Receptors, Calcitriol, Rats
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