The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Isolation and characterization of DNA from the plasma of cancer patients
Abstract
Unlabelled Ten out of 37 patients with advanced malignant diseases were found to have extractable amounts of DNA in their plasma whereas no DNA could be detected in 50 normal controls. After its purification from the original nucleoprotein complex, DNA plasma levels ranging from 0.15 to 12 micrograms/ml were measured, the lowest concentration detectable with our method being 0.1 microgram/ml. Knowing from recovery experiments performed with 32P-DNA that the loss of DNA during the extraction procedure is about 65%, the real concentration of DNA in the plasma corresponds to about 3 times the given figures. The purified DNA was shown to be double-stranded and composed of fractions ranging from 21 kb to less than 0.5 kb, as determined by agarose gel electrophoresis. All these fractions hybridized with a 32P-labelled human DNA probe indicating the human origin of the bulk of the circulating DNA.In conclusion the finding of extractable amounts of DNA in the plasma of 27% of the investigated cancer patients, and its absence from the controls, suggests some correlation with malignancy.
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“The discovery of cell free DNA (cfDNA) in blood plasma has provided a simple source of genetic material for pre-natal and tumour diagnosis (13–18) that could potentially enable routine minimally invasive sampling for subsequent CNV analysis.”
From Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation (2012) · cites this paper as [14]
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Cites
Rigby, P. W. J., Dieckmann, M., Rhodes, C., & Berg, P. R. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90052-3 · Cited by 11,400
(1977) · PubMed · Cited by 1,801
Spontaneous release of DNA by human blood lymphocytes as shown in an in vitro system.
Anker, P., Stroun, M., & Maurice, P. A. (1975) · PubMed · Cited by 234
Self-citationExcretion of Deoxyribonucleic Acid by Lymphocytes Stimulated with Phytohemagglutinin or Antigen
ROGERS, J. C., Boldt, D. H., Kornfeld, S. A., Skinner, S. A. M., & VALERI, C. R. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.7.1685 · Cited by 158
Circulating Nucleic Acids in Higher Organisms
Stroun, M., Anker, P., Maurice, P. A., & Gahan, P. B. (1977) · International review of cytology · doi:10.1016/s0074-7696(08)60225-9 · Cited by 106
Self-citationCirculating DNA in systemic lupus erythematosus. Isolation and characterization.
Steinman, C. R. (1984) · Journal of Clinical Investigation · doi:10.1172/jci111278 · Cited by 99
Wieczorek, A. J., Rhyner, C., & Block, L. H. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.10.3455 · Cited by 97
Circulating DNA in systemic lupus erythematosus
Steinman, C. R. (1979) · The American Journal of Medicine · doi:10.1016/0002-9343(79)90789-7 · Cited by 95
Aggarwal, S. K., Wagner, R. W., McAllister, P. K., & Rosenberg, B. H. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.928 · Cited by 79
Nucleic acids spontaneously released by living frog auricles
Stroun, M., & Anker, P. (1972) · Biochemical Journal · doi:10.1042/bj1280100pb · Cited by 69
Self-citation
Research works printed but not linked: 1
- Carpentier, 1981, The presence of circulating DNA in patients with acute or chronic leukemia: relation to serum anti-DNA antibodies and Clq binding activity, Human Lymphocyte Differentiation, 1, 93
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A view on drug resistance in cancer
Vasan, N., Baselga, J., & Hyman, D. M. (2019) · Nature · doi:10.1038/s41586-019-1730-1 · Cited by 2,770
Direct detection of early-stage cancers using circulating tumor DNA
Phallen, J. A., Sausen, M., Adleff, V., Leal, A., Hruban, C., White, J. R., Anagnostou, V., Fiksel, J., Cristiano, S., Papp, E., et al. (2017) · Science Translational Medicine · doi:10.1126/scitranslmed.aan2415 · Cited by 1,149
Origins, structures, and functions of circulating DNA in oncology
Thierry, A. R., El Messaoudi, S., Gahan, P. B., Anker, P., & Stroun, M. (2016) · Cancer and Metastasis Reviews · doi:10.1007/s10555-016-9629-x · Cited by 818
Self-citationHigh-intensity sequencing reveals the sources of plasma circulating cell-free DNA variants
Razavi, P., Li, B. T., Brown, D. N., Jung, B., Hubbell, E. A., Shen, R., Abida, W. M., Juluru, K., de Bruijn, I., Hou, C., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0652-7 · Cited by 808
Advances in bladder cancer biology and therapy
Tran, L. L., Xiao, J.-F., Agarwal, N., Duex, J. E., & Theodorescu, D. (2020) · Nature reviews. Cancer · doi:10.1038/s41568-020-00313-1 · Cited by 758
Metadata
- Authors
- Stroun, M., Anker, P., Lyautey, J., Lederrey, C., & Maurice, P. A.
- Affiliations
- University of Geneva (CH)
- University Hospital of Geneva (CH)
- Venue
- European Journal of Cancer and Clinical Oncology
- Publisher
- Elsevier BV
- Published
- 1987-06
- Volume
- 23
- Issue
- 6
- Pages
- 707-712
- ISSN
- 0277-5379 (ISSN-L)
- OpenAlex
- W2058440954
- Cited by
- 373
- References
- 17
- Citations by year
- 2026: 8 · 2025: 9 · 2024: 7 · 2023: 18 · 2022: 16 · 2021: 21 · 2020: 21 · 2019: 29 · 2018: 27 · 2017: 13 · 2016: 13 · 2015: 10 · 2014: 15 · 2013: 11 · 2012: 18
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Cancer and Clinical Oncology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- circulating tumor DNA, plasma DNA, cancer patients, DNA extraction, DNA purification, double-stranded DNA, DNA fragment size distribution, DNA hybridization, malignancy marker
- MeSH terms
- DNA, Neoplasm, Electrophoresis, Agar Gel, Humans, Neoplasms, Nucleic Acid Hybridization
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