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Cited by 235RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyNuclear Structure and Function

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The assembly of prokaryotic ribosomes

Nierhaus, K.H.·1991·Biochimie
DOI 10.1016/0300-9084(91)90054-5
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Abstract

The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). The success of in vitro analyses is particularly remarkable in view of ionic and temperature requirements of the total reconstitution which differ significantly from the in vivo conditions. Features of the in vivo assembly are surveyed, however, the focal point is a description of experimental strategies and results concerning the in vitro assembly of ribosomes.

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  • The hierarchical assembly pathways determined in the kinetic model for ribosome biogenesis were consistent with the in vitro assembly map assigning primary, secondary, and tertiary rproteins.

    “The hierarchical pathways were consistent with the in vitro assembly map assigning primary, secondary, and tertiary rproteins.”

    From Bringing the genetically minimal cell to life on a computer in 4D (2026) · cites this paper as [102]

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Metadata

Authors
Nierhaus, K. H.
Affiliations
Max Planck Institute for Molecular Genetics (DE)
Corresponding author
Nierhaus, K.H.
Venue
Biochimie
Publisher
Elsevier BV
Published
1991-06
Volume
73
Issue
6
Pages
739-755
ISSN
  • 0037-9042 (ISSN-L)
  • 0300-9084
DOI
10.1016/0300-9084(91)90054-5
OpenAlex
W2087416703
Cited by
235
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 5 · 2023: 20 · 2022: 6 · 2021: 10 · 2020: 2 · 2019: 7 · 2018: 6 · 2017: 3 · 2016: 4 · 2015: 13 · 2014: 9 · 2013: 10 · 2012: 10
FWCI
0.25
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Biochimie (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
ribosome assembly, prokaryotic ribosomes, rRNA processing, in vitro reconstitution, assembly map, rate-determining step
MeSH terms
Animals, Mitochondria, Models, Biological, Prokaryotic Cells, Ribosomal Proteins, Ribosomes, RNA, Ribosomal, Chlamydomonas reinhardtii, Macromolecular Substances

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