The effect of EDTA and serum on endogenous porphyrin accumulation and photodynamic sensitization of human K562 leukemic cells
Abstract
The interrelationship between the effect of serum on the induction of porphyrin synthesis, intracellular porphyrin accumulation and photodynamic sensitization of human K562 cells is described. Endogenous porphyrins, synthesized from supplemented 5-amino levulinic acid (5-ALA), were shown to accumulate in the cells, while an addition of serum triggered porphyrin translocation from the cell to the serum. In order to enhance porphyrin accumulation in the cells themselves, they were further stimulated by EDTA, which in combination with 5-ALA reduces Fe++ cellular content. The higher porphyrin cellular content under EDTA and 5-ALA induction was exploited to photoinactivate the human leukemic cells by more then 3 orders of magnitude.
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EDTA is the first chelator shown to increase PpIX levels in cells and sensitize cells to ALA-PDT.
“Non-specific and membrane-impermeable metal ion chelator ethylenediaminetetraacetic acid (EDTA) is the first chelator shown to increase PpIX levels in cells [83] and sensitize cells to ALA-PDT [84].”
From 5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy (2023) · cites this paper as [84]
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- Authors
- Hanania, J., & Malik, Z.
- Affiliations
- Bar-Ilan University (IL)
- Corresponding author
- Malik, Zvi
- ORCID
- Malik, Zvi
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 1992-08
- Volume
- 65
- Issue
- 2
- Pages
- 127-131
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W2074699077
- Cited by
- 113
- References
- 0
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 1 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 4 · 2013: 4 · 2012: 1
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
- Physical Sciences › Materials Science › Materials Chemistry › Porphyrin and Phthalocyanine Chemistry
- Keywords
- porphyrin accumulation, photodynamic therapy, K562 cells, leukemia, EDTA, serum, 5-aminolevulinic acid, porphyrin synthesis, iron depletion, photoinactivation, endogenous porphyrins
- MeSH terms
- Aminolevulinic Acid, Blood, Cell Line, Cell Survival, Culture Media, Edetic Acid, Humans, Kinetics, Light, Porphyrins, Ultraviolet Rays, Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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