Membrane responses and changes in cAMP levels in Aplysia sensory neurons produced by serotonin, tryptamine, FMRFamide and small cardioactive peptideB (SCPB)
Abstract
While recent evidence indicates a role for serotonin (5-HT) in modulating the defensive tail-withdrawal reflex in Aplysia, little information exists concerning the specificity of these 5-HT effects. As a first-step in addressing this issue we have examined the dose-response relationship for one aspect of the 5-HT modulation (enhancement of cAMP levels in isolated clusters of sensory neurons) and compared the effects of 5-HT with three potential neurotransmitters: tryptamine, FMRFamide (Phe-Met-Arg-Phe-NH2) and small cardioactive peptideB (SCPB). Cyclic adenosine monophosphate (cAMP) levels were enhanced as a graded function of the concentration of 5-HT with an EC50 of 14 microM. At a concentration of 5 microM, both 5-HT and SCPB produced nearly identical increases in the cAMP content of sensory neurons. In contrast, 5 microM tryptamine or 5 microM FMRFamide had little or no effect on cAMP levels. We also examined the effects of these agents on membrane currents and membrane conductance. Both 5-HT and SCPB produced an inward current associated with a decrease in input conductance. Tryptamine had little or no effect, while FMRFamide produced a response opposite to that of 5-HT and SCPB; an outward current associated with an increase in membrane conductance.
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The PKA and ERK cascades are necessary to induce long-term synaptic facilitation (LTF) in Aplysia.
“Two biochemical cascades required for LTF are those that mediate activation of protein kinase A (PKA)12–15 and the MAP kinase isoform termed extracellular signal-regulated kinase (ERK)16–18.”
From Computational design of enhanced learning protocols (2012) · cites this paper as [14]
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References 18 · Cited by 115
- Authors
- Ocorr, K. A., & Byrne, J. H.
- Corresponding author
- Byrne, John H.
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1985-04
- Volume
- 55
- Issue
- 2
- Pages
- 113-118
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2065490230
- Cited by
- 115
- References
- 18
- Citations by year
- 2013: 1 · 2012: 1
- FWCI
- 4.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health (MH09014)
- National Institute of Neurological Disorders and Stroke (NS19895)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- serotonin, tryptamine, FMRFamide, cyclic AMP, sensory neurons, Aplysia, membrane currents, membrane conductance, input conductance, tail withdrawal reflex, dose-response relationship, neurotransmitter specificity
- MeSH terms
- Cyclic AMP, Animals, Aplysia, Ganglia, Membrane Potentials, Neurons, Afferent, Neuropeptides, Oligopeptides, Serotonin, Tryptamines, FMRFamide, In Vitro Techniques
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