In vitro propagation of some olive (Olea europaea sativa L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos

Different fundamental formulations are required for olive genotypes.

“Different fundamental formulations are required for olive genotypes [10, 15]”

Apical dominance and oxidized tannins from phenolic compounds in semi-solid media inhibit normal regeneration in olive.

“Apical dominance and oxidized tannins from phenolic compounds in semi-solid media inhibited the normal regeneration in olive [9, 10].”

In vitro culture of olive has a genotype-dependent low micropropagation rate that needs improvement in established and newly released cultivars.

“In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars.”

Callus formation is mainly dependent on cultivars, media and PGRs.

“Callus formation is mainly dependent on cultivars, media and PGRs [9, 10, 21].”

OM is the best medium for olive micropropagation, high in Ca, Mg, S, Cu and Zn.

“Analysis of olive shoot tip (4–5 cm) by Rugini [10] resulted in introducing the best medium for olive micropropagation called OM which is high in Ca, Mg, S, Cu and Zn elements.”