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Cited by 76Nerve injury and regenerationNeuropeptides and Animal PhysiologyCongenital gastrointestinal and neural anomalies

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GAP-43 immunoreactivity is widespread in the autonomic neurons and sensory neurons of the rat

Stewart, H.J.S., Cowen, T., Curtis, R., Wilkin, G.P., Mirsky, R., Jessen, K.R.·1992·Neuroscience
DOI 10.1016/0306-4522(92)90175-2
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Abstract

GAP-43 is a membrane-bound phosphoprotein generally associated with axon growth during development and regeneration. Using immunohistochemical and immunoblotting techniques this study shows that GAP-43 is expressed extensively in the unperturbed adult autonomic nervous system. Strong immunoreactivity was seen in the developing and mature enteric subdivision of the autonomic nervous system and in nerves of the iris and various blood vessels. The presence of GAP-43 immunoreactivity in varicose nerve fibres, and a comparison of the labelling pattern of GAP-43 with the nerve associated marker PGP 9.5 suggests that GAP-43 is present in most or all autonomic nerve fibres in these organs. Immunoblotting of gut samples on 10% polyacrylamide gels revealed a single band of approximately 45,000 mol. wt that co-migrated with pure central nervous system GAP-43. Surgical sympathectomy experiments resulting in almost complete elimination of sympathetic fibres did not markedly affect the pattern of GAP-43 immunoreactivity in the iris, indicating that GAP-43 is expressed not only in sympathetic nerves but also in parasympathetic and sensory fibres. These findings show that GAP-43 is expressed extensively in autonomic nerves of the adult rat, at levels comparable to those seen during development. High levels of GAP-43 are not therefore restricted to development and regeneration in this part of the nervous system.

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  • GAP-43 is expressed in both the CNS and the ENS; it is found in high levels throughout the lifespan in the hippocampus, as well as in the myenteric and submucous ganglia.

    “An important neurodevelopment and plasticity-related protein growth-associated protein-43 (GAP-43) is expressed in both the CNS and the ENS; it is found in high levels throughout the lifespan in the hippocampus (Simmons et al., 2008), as well as in the myenteric and submucous ganglia (Giaroni et al., 1999;Stewart et al., 1992).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Stewart et al., 1992

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Authors
Stewart, H. J. S., Cowen, T., Curtis, R., Wilkin, G. P., Mirsky, R., & Jessen, K. R.
Affiliations
  • University College London (GB)
  • Imperial College London (GB)
  • The Royal Free Hospital (GB)
Corresponding author
Stewart, H.J.S.
ORCID
Jessen, K.R.
Venue
Neuroscience
Publisher
Elsevier BV
Published
1992-04
Volume
47
Issue
3
Pages
673-684
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/0306-4522(92)90175-2
OpenAlex
W1988843477
Cited by
76
References
0
Citations by year
2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2014: 1 · 2012: 2
FWCI
3.23
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Health Sciences › Medicine › Surgery › Congenital gastrointestinal and neural anomalies
Keywords
GAP-43, neurofilaments, autonomic nervous system, enteric nervous system, sensory neurons, sympathetic nerves, parasympathetic nerves, varicose nerve fibers, PGP 9.5, sympathectomy, axon growth, adult nervous system
MeSH terms
Animals, Autonomic Nervous System, Catecholamines, Cholinergic Fibers, Electrophoresis, Polyacrylamide Gel, Immunohistochemistry, Male, Membrane Glycoproteins, Myenteric Plexus, Nerve Tissue Proteins, Neurons, Neurons, Afferent, Phosphoproteins, Rats, Inbred Strains, Sympathectomy, Blotting, Northern, GAP-43 Protein, Rats

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