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High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method
Abstract
Abstract A new, simple procedure is described for the production of 5 nm colloidal gold/secondary antibody reagents. Utilizing them with antitubulin shows 1) that they can be used for high resolution ultrastructural localization studies and 2) that this can be done with retention of satisfactory preservation of cell structure. The same, simple procedure can be used to prepare 20 nm colloidal gold/antibody reagents. These can be used for the high resolution light microscopic visualization of microtubules in interphase and mitotic cells. Colloidal gold labelled serum or monoclonal antibodies can be used in a new, general purpose immunocytochemical technique: the IGS (immuno gold staining) method.
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Cited by
Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy
Martell, J. D., Deerinck, T. J., Sancak, Y., Poulos, T. L., Mootha, V. K., Sosinsky, G. E., Ellisman, M. H., & Ting, A. Y. (2012) · Nature Biotechnology · doi:10.1038/nbt.2375 · Cited by 722
Osborn, M., & Weber, K. (1982) · Methods in Cell Biology · doi:10.1016/s0091-679x(08)60650-0 · Cited by 382
Grabber, M. B., & Kreutzberg, G. W. (1986) · Journal of Neurocytology · doi:10.1007/bf01611438 · Cited by 255
Metadata
- Authors
- DEMEY, J., MOEREMANS, M., GEUENS, G., NUYDENS, R., & DEBRABANDER, M.
- Venue
- Cell Biology International Reports
- Publisher
- Wiley
- Published
- 1981-09
- Volume
- 5
- Issue
- 9
- Pages
- 889-899
- ISSN
- 0309-1651 (ISSN-L)
- OpenAlex
- W2072543637
- Cited by
- 307
- References
- 0
- FWCI
- 10.02
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method. https://app.cochat.ai/papers/10.1016/0309-1651(81)90204-6
- The paper
- DEMEY, J., MOEREMANS, M., GEUENS, G., NUYDENS, R., & DEBRABANDER, M. (1981). High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method. Cell Biology International Reports. https://doi.org/10.1016/0309-1651(81)90204-6
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
