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Cited by 63Cancer, Hypoxia, and MetabolismMetabolism, Diabetes, and CancerCancer and biochemical research

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Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma

Kenzaburo, Tani, Yoshida, Michihiro C., Hitoshi, Satoh, Keiji, Mitamura, Tamio, Noguchi, Takehiko, Tanaka, Hisaichi, Fujii, Shiro, Miwa·1988·Gene
DOI 10.1016/0378-1119(88)90515-x
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Abstract

Two overlapping clones, covering the entire coding sequence of human M2-type pyruvate kinase (PK) cDNA, were isolated and sequenced. Nucleotide sequencing results showed that they contained the 109-bp 5'-untranslated region, the 1593-bp coding region and the 585-bp 3'-untranslated region. Nucleotide sequence homology was 90% and 69% with rat M2-type and L-type PK cDNA, respectively. In situ hybridization using the human M2-type PK cDNA probe disclosed that the gene for M2-type PK is located at band q22 on chromosome 15. Northern blot analysis with RNA from human hepatoma demonstrated that M2-type PK was predominantly expressed in hepatoma cells, whereas L-type PK was preferentially expressed in the non-tumor portion of the liver.

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Research works printed but not linked: 26

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Metadata

Authors
Kenzaburo, T., Yoshida, M. C., Hitoshi, S., Keiji, M., Tamio, N., Takehiko, T., Hisaichi, F., & Shiro, M.
Affiliations
Hokkaido University (JP)
ORCID
Yoshida, Michihiro C.
Venue
Gene
Publisher
Elsevier BV
Published
1988-12
Volume
73
Issue
2
Pages
509-516
ISSN
0378-1119 (ISSN-L)
DOI
10.1016/0378-1119(88)90515-x
OpenAlex
W2081428726
Cited by
63
References
0
Citations by year
2025: 1 · 2024: 1 · 2023: 2 · 2021: 1 · 2019: 1 · 2018: 2 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 3
FWCI
1.64
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Gene (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology
  • Ministry of Health, Labour and Welfare
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
Keywords
L-type pyruvate kinase, cDNA cloning, gene mapping, hepatocellular carcinoma, gene expression, liver, sequence homology
MeSH terms
Amino Acid Sequence, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 15, Cloning, Molecular, DNA, Recombinant, Genes, Carcinoma, Hepatocellular, Humans, Isoenzymes, Liver Neoplasms, Molecular Sequence Data, Nucleic Acid Hybridization, Pyruvate Kinase, Blotting, Southern, Restriction Mapping

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CoChat. (2026). Citation check: Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma. https://app.cochat.ai/papers/10.1016/0378-1119(88)90515-x
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Kenzaburo, T., Yoshida, M. C., Hitoshi, S., Keiji, M., Tamio, N., Takehiko, T., Hisaichi, F., & Shiro, M. (1988). Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma. Gene. https://doi.org/10.1016/0378-1119(88)90515-x