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Cited by 26Herpesvirus Infections and TreatmentsAnimal Virus Infections Studiesinterferon and immune responses

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Immune responses of specific pathogen free foals to EHV-1 infection.

Chong, Y.C., Duffus, W.P.H.·1992·Veterinary Microbiology
DOI 10.1016/0378-1135(92)90146-k
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Abstract

Four foals were raised under specific pathogen free (SPF) conditions. At 3 to 4 months of age, SPF foals and 1 other non-SPF foal were intranasally inoculated with equine herpes virus type 1 (EHV-1). Clinical signs included depression, fever, inappetence and intermittent coughing. Clinical recovery was complete by seven days but high titres of virus were detected in nasal mucus for at least 10 days after inoculation. Clinical illness was less severe in the non-SPF foal. Interferon was detected in the nasal mucus of all foals from 2 days post infection (dpi), persisting until 8 or 10 dpi. ELISA antibody was detected in serum from 6 dpi. Titres continued to rise throughout the period of observation, and were slightly stimulated by re-inoculation. EHV antibody, identified as belonging to the IgM class by the double sandwich ELISA, was detected from 6 dpi. Peak IgM titres were observed between day 10 and 18, declining to base levels by day 42. Virus neutralizing antibody was detectable in serum from day 14 and rises in titre were parallel to that of total ELISA antibody. Cellular immunity in EHV-1 infected SPF horses was examined by the antibody dependent cytotoxicity (ADCC) test and the specific lymphocyte transformation test. The ability of foal neutrophils to effect ADCC decreased significantly between 3 to 10 days after inoculation. Peripheral blood mononuclear cells (PBMC) displayed reactivity towards EHV-1 antigens from about day 14, with maximum stimulation indices being obtained between 28 and 42 dpi.

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Metadata

Authors
Chong, Y. C., & Duffus, W. P. H.
Affiliations
University of Cambridge (GB)
Corresponding author
Duffus, W.P.H.
Venue
Veterinary Microbiology
Publisher
Elsevier BV
Published
1992-10
Volume
32
Issue
3-4
Pages
215-239
ISSN
0378-1135 (ISSN-L)
DOI
10.1016/0378-1135(92)90146-k
OpenAlex
W1969326874
Cited by
26
References
0
Citations by year
2024: 4 · 2022: 1 · 2021: 1 · 2015: 1 · 2014: 1 · 2013: 2
FWCI
0.00
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Herpesvirus Infections and Treatments
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
Keywords
equine herpesvirus 1, foals, immune response, interferon, IgM, virus-neutralizing antibodies, antibody-dependent cellular cytotoxicity, lymphocyte transformation, cell-mediated immunity, peripheral blood mononuclear cells, neutrophils, reinfection, nasal mucus
MeSH terms
Animals, Antibodies, Viral, Antibody-Dependent Cell Cytotoxicity, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Herpesvirus 1, Equid, Herpesviridae Infections, Horse Diseases, Horses, Immunoglobulin M, Interferons, Lymphocyte Activation, Neutralization Tests, Neutrophils, Specific Pathogen-Free Organisms

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Chong, Y. C., & Duffus, W. P. H. (1992). Immune responses of specific pathogen free foals to EHV-1 infection. Veterinary Microbiology. https://doi.org/10.1016/0378-1135(92)90146-k

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