Endothelial cell harvest for seeding vascular prostheses: The influence of technique on cell function, viability, and number
Open accessAbstract
Direct seeding of endothelial cells onto synthetic vascular prostheses has become the subject of increasing surgical research during the last 5 to 7 years. The currently employed cell harvest techniques are inefficient, resulting in cell counts far below the number of cells calculated to be present on the original donor vein. We have compared two methods of enzymatic endothelial cell harvest: cannulation with flushing and eversion over a stainless steel rod. Harvested cells were plated onto tissue culture plastic and counted after 24 hours of incubation. The methods ensured that only those cells viable and functional enough to adhere to the plastic were being considered. Cells were identified as endothelial by immunohistochemical techniques applying antisera to factor VIII-related antigen. Segments of normal vein and of veins treated by each technique were viewed with a scanning electron microscope. Cannulation was the superior method, providing greater numbers of viable, functional cells. The eversion technique was unreliable and probably injurious to endothelial cells.
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Both HUVECs and EA. hy926 cells were able to line the blood vessels of decellularized livers, with a significant reduction in platelet adhesion and clot formation during ex vivo blood perfusion, and demonstrated the ability to withstand blood flow after in vivo transplantation in pigs.
“Both cell types were able to line the blood vessels of decellularized livers, with a significant reduction in platelet adhesion and clot formation during ex vivo blood perfusion and demonstrated the ability to withstand blood flow after in vivo transplantation in pigs.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [235]
Endothelial cells have limited application due to donor variability, technical challenges of isolation, limited proliferative capacity, senescence, and heterogeneity.
“However, their application is limited due to the donor variability, technical challenges of isolation, limited proliferative capacity, senescence, and heterogeneity [235,236].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [235]
HUVECs and EA. hy926 cells have been extensively used for reendothelization of decellularized livers due to relative ease of accessibility, non-invasive harvesting from medical waste, and high yield following isolation.
“Cell lines, including HUVECs and EA. hy926 cells, have been extensively used for reendothelization of decellularized livers due to their relative ease of accessibility and the non-invasive harvesting from medical waste, in addition to a high yield following isolation [10,13,14,235].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [235]
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Hussein, K. H., Park, K.-M., Yu, L., Song, S.-H., Woo, H.-M., & Kwak, H.-H. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.12.029 · Cited by 44 · see citing works
Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“However, their application is limited due to the donor variability, technical challenges of isolation, limited proliferative capacity, senescence, and heterogeneity [235,236].”
Background
- Authors
- Bourke, B. M., Roche, W. R., & Appleberg, M.
- Affiliations
- The University of Sydney (AU)
- Venue
- Journal of Vascular Surgery
- Publisher
- Elsevier BV
- Published
- 1986-09
- Volume
- 4
- Issue
- 3
- Pages
- 257-263
- ISSN
- 0741-5214 (ISSN-L)
- OpenAlex
- W1984274839
- Cited by
- 22
- References
- 0
- Citations by year
- 2024: 1 · 2022: 1 · 2019: 1 · 2018: 1
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Vascular Surgery (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- direct seeding, vascular grafts, cell viability, cell function, factor VIII-related antigen, cell number
- MeSH terms
- Animals, Blood Vessel Prosthesis, Cell Count, Cell Separation, Cell Survival, Cytological Techniques, Endothelium, Female, Histocytochemistry, Jugular Veins, Microscopy, Electron, Scanning, Sheep
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