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Cited by 51Estrogen and related hormone effectsGrowth Hormone and Insulin-like Growth FactorsEffects and risks of endocrine disrupting chemicals

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Dexamethasone attenuates the estradiol-induced increase of IGF-I mRNA in the rat uterus

Sahlin, Lena·1995·The Journal of Steroid Biochemistry and Molecular Biology
DOI 10.1016/0960-0760(95)00152-p
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Abstract

In recent years growth factors, e.g. insulin-like growth factor-I (IGF-I), transforming growth factor-alpha (TGF-alpha) and epidermal growth factor (EGF), have been considered as mediators of estradiol-stimulated growth in the uterus. In the liver, dexamethasone (Dex) has been shown to inhibit the IGF-I mRNA increase induced by growth hormone (GH). In the present study the influence of Dex on estradiol-stimulated induction of uterine IGF-I mRNA was examined. The concentration of IGF-I mRNA in the uterus and liver was monitored, as well as the levels of ER mRNA and estrogen receptor (ER). Since it has been previously shown that the maximal induction of uterine IGF-I mRNA after estradiol (E2) stimulation occurs after 21-24 h, Dex was administered to ovariectomized (ovx) rats 3 h before an E2 injection and 24 h before sacrifice. There was a significant decrease in IGF-I mRNA in the Dex+E2 treated rats compared to the rats given E2 only. In both groups an increase was seen compared to the level in the ovx control group. The uterine ER mRNA levels in E2 and Dex+E2 treated animals were significantly elevated compared to the ovx control. There were no significant changes in uterine ER content after hormone treatment compared to the level in ovx control rats. In the liver no effects on IGF-I mRNA were detected. Hepatic ER mRNA was significantly increased in the E2 treated group, compared to both the ovx control group and the animals that received Dex+E2. The hepatic ER level was also increased in the E2 treated group compared to the ovx control and the group which received Dex+E2. In conclusion, Dex does attenuate the estrogen-induced uterine IGF-I mRNA increase in ovx rats. In addition to this, Dex was found to inhibit the estrogen-induced increase in ER and ER mRNA in the liver of ovx rats.

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Cited by

Metadata

Authors
Sahlin, L.
Affiliations
Karolinska University Hospital (SE)
Corresponding author
Sahlin, Lena
ORCID
Sahlin, Lena
Venue
The Journal of Steroid Biochemistry and Molecular Biology
Publisher
Elsevier BV
Published
1995-10
Volume
55
Issue
1
Pages
9-15
ISSN
0960-0760 (ISSN-L)
DOI
10.1016/0960-0760(95)00152-p
OpenAlex
W1985461422
Cited by
51
References
0
Citations by year
2026: 1 · 2025: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2015: 3
FWCI
2.06
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
Keywords
dexamethasone, estradiol, insulin-like growth factor I, IGF-I mRNA, uterus, liver, estrogen receptor, ovariectomized rats, uterine growth
MeSH terms
Animals, Dexamethasone, Estradiol, Estrogen Antagonists, Female, Gene Expression Regulation, Glucocorticoids, Insulin-Like Growth Factor I, Liver, Ovariectomy, Receptors, Estradiol, RNA, Messenger, Growth Hormone, Uterus, Rats, Sprague-Dawley, Rats

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