The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
The mitochondrion as a primary site of action of glucocorticoids: the interaction of the glucocorticoid receptor with mitochondrial DNA sequences showing partial similarity to the nuclear glucocorticoid responsive elements
Abstract
Six mitochondrial genome sequences, showing strong similarity to the glucocorticoid responsive element consensus sequence (GRE), four localized within the cytochrome c oxidase (COX) subunit I and II genes (GREs I-IV) and two within the D-loop region (GREs a and b) have been examined as binding sites of glucocorticoid receptor (GR) from rat liver cytosol. Purified GR from rat liver cytosol binds with high specificity to all potential mitochondrial GREs, as shown by filter retention and gel shift assays. Specific binding of protein(s), present in a mitochondrial extract from dexamethasone-induced mice, to all six putative mitochondrial GREs was also documented by the same methodology. Both purified GR and protein(s) from mitochondrial extract give the same band in the gel retardation assay. Using monospecific anti-glucocorticoid receptor polyclonal antibody (EP), a supershift of the gel retarded protein-DNA band was obtained. These results demonstrate that the mitochondrial genome sequences examined have characteristics of GREs, since they show the capacity to specifically bind the respective receptor protein. These findings support the hypothesis that the mitochondrial genome is a primary site of action of steroid and thyroid hormones (Sekeris C.E.: The mitochondrial genome: a possible primary site of action of steroid hormones, In vivo 4 (1990) 317-320).
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“these elements have been found in mtDNA accompanied by oestrogen and androgen receptors (Demonacos et al., 1995; Demonacos et al., 1996).”
From Mitochondrial Activity and Dynamics Changes Regarding Metabolism in Ageing and Obesity (2017) · cites this paper as Demonacos et al., 1995
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318
Sequence and organization of the human mitochondrial genome
Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., et al. (1981) · Nature · doi:10.1038/290457a0 · Cited by 9,740
The Steroid and Thyroid Hormone Receptor Superfamily
Evans, R. M. (1988) · Science · doi:10.1126/science.3283939 · Cited by 7,394
Gene regulation by steroid hormones
Beato, M. (1989) · Cell · doi:10.1016/0092-8674(89)90237-7 · Cited by 3,383
Garner, M. M., & Revzin, A. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.13.3047 · Cited by 2,021
STEROID RECEPTOR REGULATED TRANSCRIPTION OF SPECIFIC GENES AND GENE NETWORKS
Yamamoto, K. R. (1985) · Annual Review of Genetics · doi:10.1146/annurev.ge.19.120185.001233 · Cited by 1,850
Regulation of Metabolic Processes in Mitochondria
(1967) · Food and Cosmetics Toxicology · doi:10.1016/s0015-6264(67)83077-3 · Cited by 1,178
Nuclear receptors enhance our understanding of transcription regulation
Green, S., & Chambon, P. (1988) · Trends in Genetics · doi:10.1016/0168-9525(88)90108-4 · Cited by 1,042
Chandler, V. L., Maler, B. A., & Yamamoto, K. R. (1983) · Cell · doi:10.1016/0092-8674(83)90430-0 · Cited by 697
Payvar, F., DeFranco, D. B., Firestone, G. L., Edgar, B. A., Wränge, Ö., Okret, S., Gustafsson, J.-Å., & Yamamoto, K. R. (1983) · Cell · doi:10.1016/0092-8674(83)90171-x · Cited by 678
Scheidereit, C., Geisse, S., Westphal, H. M., & Beato, M. (1983) · Nature · doi:10.1038/304749a0 · Cited by 601
Kadenbach, B., Jarausch, J., Hartmann, R., & Merle, P. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90586-9 · Cited by 431
Inhibition of Electron and Energy Transfer in Mitochondria
Chance, B., & Hollunger, G. (1963) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)84014-0 · Cited by 366
THE MITOCHONDRIAL PROTEIN IMPORT APPARATUS
Pfanner, N., & Neupert, W. (1990) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.59.070190.001555 · Cited by 308
Purification of the glucocorticoid receptor from rat liver cytosol.
Wränge, Ö., Carlstedt‐Duke, J., & Gustafsson, J. A. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)86842-4 · Cited by 238
Gustafsson, R. G., Tata, J. R., Lindberg, O. R., & Ernster, L. (1965) · The Journal of Cell Biology · doi:10.1083/jcb.26.2.555 · Cited by 216
Hood, D. A., Zak, R., & Pette, D. (1989) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1989.tb14551.x · Cited by 161
Glucocorticoid receptors recognize DNA sequences in and around murine mammary tumour virus DNA.
Geisse, S., Scheidereit, C., Westphal, H. M., Hynes, N. E., Groner, B., & Beato, M. (1982) · The EMBO Journal · doi:10.1002/j.1460-2075.1982.tb01363.x · Cited by 148
Wiesner, R. J., Kurowski, T. T., & Zak, R. (1992) · Molecular Endocrinology · doi:10.1210/mend.6.9.1331777 · Cited by 146
CORTISONE-INDUCED ALTERATIONS IN MITOCHONDRIAL FUNCTION AND STRUCTURE
Kimberg, D. V., Loud, A. V., & Wiener, J. (1968) · The Journal of Cell Biology · doi:10.1083/jcb.37.1.63 · Cited by 98
Suzuki, H., Hosokawa, Y., Nishikimi, M., & Ozawa, T. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)52248-1 · Cited by 97
On the mechanism of hormone action
Congote, L. F., Sekeris, C. E., & Karlson, P. (1969) · Experimental Cell Research · doi:10.1016/0014-4827(69)90023-8 · Cited by 92
Self-citationBeato, M., Biesewig, D., Braendle, W. L., & Sekeris, C. E. (1969) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(69)90399-7 · Cited by 92
Self-citationImport of the glucocorticoid receptor into rat liver mitochondria in vivo and in vitro
Demonacos, C., Tsawdaroglou, N. C., Djordjević-Marković, R., Papalopoulou, M. A., Galanopoulos, V. K., Papadogeorgaki, S., & Sekeris, C. E. (1993) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(93)90231-k · Cited by 81
Self-citationItallie, C. M. V., & Dannies, P. S. (1988) · Molecular Endocrinology · doi:10.1210/mend-2-4-332 · Cited by 78
Itallie, C. M. V. (1990) · Endocrinology · doi:10.1210/endo-127-1-55 · Cited by 64
Wiesner, R. J. (1992) · Molecular Endocrinology · doi:10.1210/me.6.9.1458 · Cited by 63
The mitochondrial genome: a possible primary site of action of steroid hormones.
Sekeris, C. E. (1992) · PubMed · Cited by 62
Self-citationCo-ordinate expression of cytochrome c oxidase subunit III and VIc mRNAs in rat tissues
Hood, D. A. (1990) · Biochemical Journal · doi:10.1042/bj2690503 · Cited by 56
Gagnon, J., Kurowski, T. T., Wiesner, R. J., & Zak, R. (1991) · Molecular and Cellular Biochemistry · doi:10.1007/bf02424572 · Cited by 54
Kadowaki, T., & Kitagawa, Y. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)81354-1 · Cited by 44
Glucocorticoid-stimulated utilization of substrates in hepatic mitochondria
Wakat, D. K., & Haynes, R. C. (1977) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(77)90466-0 · Cited by 40
Koenig, H., Goldstone, A. D., & Lu, C. (1980) · Biochemical Journal · doi:10.1042/bj1920349 · Cited by 37
In vivo Cortisol Action on RNA Synthesis in Rat Liver Nuclei and Mitochondria
Mansour, A. M., & Nass, S. (1970) · Nature · doi:10.1038/228665a0 · Cited by 37
Dexamethasone treatment increases mitochondrial RNA synthesis in a rat hepatoma cell line.
Itallie, C. M. V. (1992) · Endocrinology · doi:10.1210/endo.130.2.1370790 · Cited by 37
The stimulation of hepatic oxidative phosphorylation following dexamethasone treatment of rats
Allan, E. H., Chisholm, A. B., & Titheradge, M. A. (1983) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(83)90225-6 · Cited by 36
Yu, F. L., & Feigelson, P. (1970) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(70)90014-6 · Cited by 33
Inhibition of mitochondrial respiration by progesterone and an azasteroid
Varricchio, F. E., & Sanadi, D. R. (1967) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(67)90023-9 · Cited by 31
Presence of glucocorticoid responsive elements in the mitochondrial genome.
Im, I., Tsawdaroglou, N. H., & Sekeris, C. E. (1989) · PubMed · Cited by 31
Self-citationPresence of Glucocorticoid Responsive Elements in the Mitochondrial Genome
Ioannou, I. M., Tsawdaroglou, N. H., & Sekeris, C. E. (1988) · National Hellenic Research Foundation Helios Repository (National Hellenic Research Foundation) · Cited by 20
Self-citationRNA SYNTHESIS IN RAT LIVER AFTER CORTISOL TREATMENT: A POSSIBLE MITOCHONDRIAL-NUCLEAR RELATIONSHIP
Mansour, A. M., & Nass, S. (1974) · European Journal of Endocrinology · doi:10.1530/acta.0.0770298 · Cited by 16
Dexamethasone treatment increases mitochondrial RNA synthesis in a rat hepatoma cell line
Itallie, C. M. V. (1992) · Endocrinology · doi:10.1210/en.130.2.567 · Cited by 15
LI, G., LIU, Y., & TSANG, S. (1994) · International Journal of Oncology · doi:10.3892/ijo.5.2.301 · Cited by 10
Westphal, H. M., & Beato, M. (1981) · FEBS Letters · doi:10.1016/0014-5793(81)80133-0 · Cited by 9
Martin, A. D., & Titheradge, M. A. (1984) · Biochemical Journal · doi:10.1042/bj2220379 · Cited by 9
Tsiriyotis, C., Spandidos, D. A., & Sekeris, C. E. (1993) · International Journal of Oncology · doi:10.3892/ijo.2.6.947 · Cited by 6
Self-citation
Cited by
Sorted by citations
Transcriptional paradigms in mammalian mitochondrial biogenesis and function
Scarpulla, R. C. (2008) · Physiological Reviews · doi:10.1152/physrev.00025.2007 · Cited by 1,666
Mitochondrial signal transduction
Picard, M. A., & Shirihai, O. S. (2022) · Cell Metabolism · doi:10.1016/j.cmet.2022.10.008 · Cited by 688
Mitochondria as key components of the stress response
Manoli, I., Alesci, S., Blackman, M. R., Su, Y. A., Rennert, O. M., & Chrousos, G. P. (2007) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2007.04.004 · Cited by 394
Iuchi, T., Akaike, M., Mitsui, T., Ohshima, Y., Shintani, Y., Azuma, H., & Matsumoto, T. (2003) · Circulation Research · doi:10.1161/01.res.0000050588.35034.3c · Cited by 358
Mitochondrial localization of ERα and ERβ in human MCF7 cells
Chen, J. Q., Delannoy, M., Cooke, C., & Yager, J. D. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00508.2003 · Cited by 272
Metadata
- Authors
- Demonacos, C., Djordjevic-Markovic, R., Tsawdaroglou, N., & Sekeris, C. E.
- Affiliations
- National Hellenic Research Foundation (GR)
- Corresponding author
- Sekeris, C.E.
- ORCID
- Demonacos, C.
- Venue
- The Journal of Steroid Biochemistry and Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1995-10
- Volume
- 55
- Issue
- 1
- Pages
- 43-55
- ISSN
- 0960-0760 (ISSN-L)
- OpenAlex
- W2047490631
- Cited by
- 131
- References
- 46
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 5 · 2022: 7 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 5 · 2016: 7 · 2015: 7 · 2014: 3 · 2013: 4 · 2012: 9
- FWCI
- 1.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- National Hellenic Research Foundation Helios Repository (National Hellenic Research Foundation) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- glucocorticoid receptor, mitochondria, mitochondrial DNA, glucocorticoid response element, cytochrome c oxidase subunit I, cytochrome c oxidase subunit II, D-loop, dexamethasone, steroid hormone action, receptor-DNA binding, rat liver
- MeSH terms
- Animals, Base Sequence, Binding Sites, Binding, Competitive, Electron Transport Complex IV, Cytosol, Dexamethasone, DNA-Binding Proteins, DNA, Mitochondrial, Male, Mice, Inbred BALB C, Mitochondria, Liver, Molecular Sequence Data, Oligodeoxyribonucleotides, Receptors, Glucocorticoid, Regulatory Sequences, Nucleic Acid, Sequence Homology, Nucleic Acid, Consensus Sequence, Rats, Wistar, Mice, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
