Imaging of Neutral Lipids and Neutral Lipid Associated Proteins
Open accessAbstract
Intracellular fat droplets are large and have a distinct morphology, which makes their imaging at the light level simple and informative. We detail how to image the fat droplet core by metabolic labeling with fluorescent fatty acids or lipophilic fluorochromes. Further, we describe the use of indirect immunostaining to image fat droplet proteins and fat cores in the same field. We also address the use of appropriate controls for determining signal specificity and other practical considerations for optimizing image quality.
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LAMP1 also labels late endosomes and non-degradative organelles.
“Since LAMP1 also labels late endosomes and non-degradative organelles37”
From INPP5D/SHIP1 regulates endo-lysosomal function and cargo-selective phagocytosis in human microglia (2025) · cites this paper as [37]
The Discussion section, “Placing fat droplets in intracellular context” (page unavailable), discusses perilipin 3 and an endoplasmic-reticulum marker, not LAMP1. Searches for LAMP1, late endosomes, and lysosome-associated membrane protein found no passage in our copy addressing whether LAMP1 labels late endosomes or non-degradative organelles. (LLM, conf=0.76)
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Marschallinger, J., Iram, T., Zardeneta, M., Lee, S. E., Lehallier, B., Haney, M. S., Pluvinage, J. V., Mathur, V., Hahn, O., Morgens, D. W., et al. (2020) · Nature Neuroscience · doi:10.1038/s41593-019-0566-1 · Cited by 1,384 · see citing works
INPP5D/SHIP1 regulates endo-lysosomal function and cargo-selective phagocytosis in human microglia
Terzioglu, G., Karp, E. S., Heuer, S. E., Chadarevian, J. P., Ahsan, F. M., Haage, V. C., Davtyan, H., Fogo, G. M., Curle, A. J., Ashour, N., et al. (2025) · doi:10.1101/2025.10.27.684632 · Cited by 3 · see citing works
- Authors
- Harris, L.-A. L. S., Skinner, J. R., & Wolins, N. E.
- Affiliations
- Washington University in St. Louis (US)
- Venue
- Methods in Cell Biology
- Publisher
- Elsevier
- Published
- 2013
- Volume
- 116
- Pages
- 213-226
- ISSN
- 0091-679X (ISSN-L)
- PMC
- PMC3870878
- OpenAlex
- W68703424
- Cited by
- 33
- References
- 0
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 7 · 2020: 4 · 2019: 4 · 2018: 1 · 2017: 5 · 2015: 1 · 2014: 1
- FWCI
- 1.42
- Type
- book chapter
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Methods in cell biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (5r01dk088206-05)
- National Heart, Lung, and Blood Institute (T32HL007275)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01-DK088206, R01 DK088206-01A1, P30DK056341)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- neutral lipids, lipid droplets, lipid droplet imaging, lipid droplet proteins
- MeSH terms
- Inclusion Bodies, Cytoplasm, Fatty Acids, Fluorescent Dyes, Lipids, Membrane Proteins, Microscopy, Fluorescence, Phosphoproteins, Lipid Metabolism
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