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Stain-Free total protein staining is a superior loading control to β-actin for Western blots
Open accessAbstract
Semi-Quantification of proteins using Western blots typically involves normalization against housekeeping genes such as β-actin. More recently, ponceau S and Coomassie blue staining have both been shown to be suitable alternatives to housekeeping genes as loading controls. Stain free total protein staining offers the advantage of no staining or destaining steps. Evaluation of the use of Stain free staining as an alternative to β-actin or the protein stain ponceau S showed that Stain free staining was superior to β-actin and as good as or better than ponceau S staining as a loading control for Western blots.
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Metadata
- Authors
- Gilda, J. E., & Gomes, A. V.
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 440
- Issue
- 2
- Pages
- 186-188
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W2081254560
- Cited by
- 331
- References
- 0
- FWCI
- 7.85
- Type
- journal article
- Language
- en
- Open access
- green
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- Gilda, J. E., & Gomes, A. V. (2013). Stain-Free total protein staining is a superior loading control to β-actin for Western blots. Analytical Biochemistry. https://doi.org/10.1016/j.ab.2013.05.027
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