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Cited by 77Bacterial Genetics and BiotechnologyMetalloenzymes and iron-sulfur proteinsProtein Structure and Dynamics

Metal site occupancy and allosteric switching in bacterial metal sensor proteins

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Guerra, Alfredo J., Giedroc, David P.·2012·Archives of Biochemistry and Biophysics
DOI 10.1016/j.abb.2011.11.021PMC PMC3312040
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Abstract

All prokaryotes encode a panel of metal sensor or metalloregulatory proteins that govern the expression of genes that allows an organism to quickly adapt to toxicity or deprivation of both biologically essential transition metal ions, e.g., Zn, Cu, Fe, and heavy metal pollutants. As such, metal sensor proteins can be considered arbiters of intracellular transition metal bioavailability and thus potentially control the metallation state of the metalloproteins in the cell. Metal sensor proteins are specialized allosteric proteins that regulate transcription as a result direct binding of one or two cognate metal ions, to the exclusion of all others. In most cases, the binding of the cognate metal ion induces a structural change in a protein oligomer that either activates or inhibits operator DNA binding. A quantitative measure of the degree to which a particular metal drives metalloregulation of operator DNA-binding is the allosteric coupling free energy, ΔGc. In this review, we summarize recent work directed toward understanding metal occupancy and metal selectivity of these allosteric switches in selected families of metal sensor proteins and examine the structural origins of ΔGc in the functional context a thermodynamic "set-point" model of intracellular metal homeostasis.

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  • Sensitivity is determined by the affinity of the metalloregulator for its cognate ion and the effects of DNA-binding on metal affinity.

    “Sensitivity is determined by the affinity of the metalloregulator for its cognate ion and the effects of DNA-binding on metal affinity (thermodynamic coupling), as discussed in detail elsewhere.26,30”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [30]

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Authors
Guerra, A. J., & Giedroc, D. P.
Affiliations
Indiana University Bloomington (US)
Corresponding author
Giedroc, David P.
ORCID
Venue
Archives of Biochemistry and Biophysics
Publisher
Elsevier BV
Published
2012-03
Volume
519
Issue
2
Pages
210-222
ISSN
0003-9861 (ISSN-L)
DOI
10.1016/j.abb.2011.11.021
PMC
PMC3312040
OpenAlex
W2085328815
Cited by
77
References
9
Citations by year
2026: 2 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 7 · 2018: 5 · 2017: 8 · 2016: 7 · 2015: 9 · 2014: 11 · 2013: 7 · 2012: 4
FWCI
0.75
Type
journal article
Language
en
Open access
Licence
Locations
  • Archives of Biochemistry and Biophysics (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
US National Institutes of Health (GM042569)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
metalloregulation, metal ion selectivity, transition metal homeostasis, transcriptional regulation, DNA binding, metalloproteins, bacterial metal homeostasis
MeSH terms
Allosteric Regulation, Bacteria, Bacterial Proteins, DNA, Bacterial, Metals

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