Differences in protein binding and cytokine release from monocytes on commercially sourced tissue culture polystyrene
Abstract
Tissue culture polystyrene (TCPS) is a ubiquitous substrate used by many researchers in the biomedical and biological sciences. Different parameters involved in the production of TCPS, including the treatment time and the use of reactive gases and chemical agents, can have a significant influence on the ultimate surface properties achieved. The assumption that they will all yield a consistent and controlled product has not proven to be true. To provide a better insight into the bioactivity differences in TCPS supplied by different manufacturers, TCPS from three different companies (Sarstedt, Wisent Corp., and Becton Dickinson (BD)) were analyzed for their surface properties, protein adsorption characteristics, and interactions with human monocytes. Marked differences were observed in terms of surface wettability and surface chemistry. Furthermore, Wisent TCPS adsorbed more than twice the amount of serum proteins compared with BD and Sarstedt TCPS. Sarstedt showed significantly more cell retention (more DNA) compared with both BD and Wisent TCPS brands over a 7 day culture period. Cytokine release from monocytes adherent on the three different TCPS also differed significantly, suggesting that the differences in the surface properties were sufficient to differentially mediate monocyte activation. These results have important implications for TCPS research use, in terms of appreciating the interpretation of the data when TCPS is used as a control substrate as well as when it is…
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Even identical formats of cell culture dishes and plates made from the same type of plastic by different manufacturers can have different surface properties as a consequence of production variability.
“Even identical formats of cell culture dishes and plates made from the same type of plastic by different manufacturers can have different surface properties as a consequence of production variability (e.g. temperature, duration of the production process, or supplier of raw materials) (Battiston et al., 2012).”
From In Vitro research Reproducibility: Keeping up High Standards (2019) · cites this paper as Battiston et al., 2012
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Santerre, J. P., Woodhouse, K., Laroche, G., & Labow, R. S. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.05.079 · Cited by 718 · see citing works
Self-citationMcNally, A. K., Jones, J. A., MacEwan, S. R., Colton, E., & Anderson, J. M. (2008) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31658 · Cited by 139 · see citing works
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In Vitro research Reproducibility: Keeping up High Standards
Hirsch, C., & Schildknecht, S. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.01484 · Cited by 161 · see citing works
Battiston, K. G., Labow, R. S., & Santerre, J. P. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.08.014 · Cited by 53 · see citing works
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- Authors
- Battiston, K. G., McBane, J. E., Labow, R. S., & Paul Santerre, J.
- Affiliations
- University of Toronto (CA)
- University of Ottawa (CA)
- ORCID
- McBane, Joanne E.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2012-01
- Volume
- 8
- Issue
- 1
- Pages
- 89-98
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2097043094
- Cited by
- 25
- References
- 2
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 4 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- NSERC/CIHR collaborative (337246/83459)
- Canadian Heart and Stroke Foundation Focus
- CIHR Strategic Training Fellowship
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- tissue culture polystyrene, monocytes, protein adsorption, cytokine production, wettability, surface chemistry, cell retention, monocyte activation, manufacturer variation, serum proteins
- MeSH terms
- Adsorption, Blood Proteins, Cell Adhesion, Cells, Cultured, Chromatography, Liquid, Humans, Monocytes, Polystyrenes, Protein Binding, Mass Spectrometry, Surface Properties, Cytokines, Wettability, Cell Culture Techniques, Photoelectron Spectroscopy
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