Differential functional effects of biomaterials on dendritic cell maturation
Open accessAbstract
The immunological outcome of dendritic cell (DC) treatment with different biomaterials was assessed to demonstrate the range of DC phenotypes induced by biomaterials commonly used in combination products. Immature DCs (iDCs) were derived from human peripheral blood monocytes, and treated with different biomaterial films of alginate, agarose, chitosan, hyaluronic acid (HA), or 75:25 poly(lactic-co-glycolic acid) (PLGA) and a comprehensive battery of phenotypic functional outcomes was assessed. Different levels of functional changes in DC phenotype were observed depending on the type of biomaterial films used to treat the DCs. Treatment of DCs with PLGA or chitosan films supported DC maturation, with higher levels of DC allostimulatory capacity, pro-inflammatory cytokine release, and expression of CD80, CD86, CD83, HLA-DQ and CD44 compared with iDCs, and lower endocytic ability compared with iDCs. Alginate film induced pro-inflammatory cytokine release from DCs at levels higher than from iDCs. Dendritic cells treated with HA film expressed lower levels of CD40, CD80, CD86 and HLA-DR compared with iDCs. They also exhibited lower endocytic ability and CD44 expression than iDCs, possibly due to an insolubilized (cross-linked) form of high molecular weight HA. Interestingly, treatment of DCs with agarose film maintained the DC functional phenotype at levels similar to iDCs except for CD44 expression, which was lower than that of iDCs. Taken together, these results can provide selection criteria for biomaterials to be used in immunomodulating applications and can inform potential outcomes of biomaterials within combination products on associated immune responses as desired by the application.
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Alginate films caused a switch toward a proinflammatory environment, with a significant increase in CD83, CD86, and HLA-DQ when compared to immature DC cytokine excretion.
“Alginate films caused a switch toward a proinflammatory environment, with a significant increase in CD83, CD86, and HLA‐DQ when compared to immature DC cytokine excretion.”
From Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes (2024) · cites this paper as [87]
Results §3.4 reports significantly higher CD83, CD86, and HLA-DQ expression in alginate-treated DCs than in immature DCs. Results §3.6 separately reports significantly higher pro-inflammatory cytokine release. The source compares marker expression with marker expression, not with immature DC cytokine excretion as the citing sentence states. (LLM, conf=0.88)
Experimental — accuracy not yet measuredBiomaterial type modulates the activation of DCs, where PLGA, alginate, and chitosan are able to induce DC maturation and invoke a shift toward a Th1 proinflammatory state, while HA and agarose create a more bioinert-like state in DCs, suppressing both Th1 and Th2 activation.
“These results indicate that biomaterial type modulates the activation of DCs, where PLGA, alginate, and chitosan are able to induce DC maturation and invoke a shift toward a Th1 proinflammatory state, while HA and agarose create a more bioinert‐like state in DCs, suppressing both Th1 and Th2 activation.”
From Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes (2024) · cites this paper as [87]
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Experimental — accuracy not yet measuredAgarose caused no significant differences in phenotype compared to immature DCs other than lowered levels of CD44.
“Agarose caused no significant differences in phenotype compared to immature DCs other than lowered levels of CD44.”
From Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes (2024) · cites this paper as [87]
The Abstract says agarose-treated dendritic cells had a functional phenotype similar to immature DCs, except for lower CD44 expression. However, Results §3.4 also notes slightly higher CD83 expression as an exception to the absence of significant differences in surface molecules. The claim therefore overlooks a reported difference. (LLM, conf=0.84)
Experimental — accuracy not yet measuredHA-treated DCs displayed a lower expression of proinflammatory cytokines, whilst also having decreased endocytic ability and CD44 expression compared to immature DCs.
“HA‐treated DCs displayed a lower expression of the aforementioned proinflammatory cytokines, whilst also having decreased endocytic ability and CD44 expression compared to immature DCs.”
From Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes (2024) · cites this paper as [87]
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References 87 · Cited by 156
- Authors
- Park, J., & Babensee, J. E.
- Affiliations
- Georgia Institute of Technology (US)
- Corresponding author
- Babensee, Julia E.
- ORCID
- Babensee, Julia E.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2012-10
- Volume
- 8
- Issue
- 10
- Pages
- 3606-3617
- ISSN
- 1742-7061 (ISSN-L)
- PMC
- PMC3970713
- OpenAlex
- W2083429131
- Cited by
- 156
- References
- 87
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 11 · 2023: 9 · 2022: 12 · 2021: 21 · 2020: 16 · 2019: 19 · 2018: 9 · 2017: 13 · 2016: 8 · 2015: 15 · 2014: 6 · 2013: 6
- FWCI
- 3.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Science Foundation
- Arthritis Foundation
- National Institutes of Health
- National Institute of Biomedical Imaging and Bioengineering (R01EB004633)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- dendritic cell maturation, alginate, agarose, chitosan, hyaluronic acid, PLGA, CD44, CD80, CD86, HLA-DR, endocytosis, inflammatory cytokines, allostimulatory capacity, immunomodulation, combination products, biomaterial selection
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Histocompatibility Antigens Class II, Biocompatible Materials, Cell Adhesion, Cell Differentiation, Cell Movement, Dendritic Cells, Endocytosis, Flow Cytometry, Fluorescence, Humans, Hyaluronic Acid, Lipopolysaccharides, Polyglycolic Acid, Receptors, Cell Surface, Sepharose, Surface Properties, T-Lymphocytes, Cytokines, Apoptosis, Inflammation Mediators, Lactic Acid, Chitosan, Cell Shape, Cell Proliferation, Transcription Factor RelA, Photoelectron Spectroscopy
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