CO2-assisted synthesis of silk fibroin hydrogels and aerogels
Abstract
Biocompatible and biodegradable porous materials based on silk fibroin (SF), a natural protein derived from the Bombyx mori silkworm, are being extensively investigated for use in biomedical applications including mammalian cell bioprocessing, tissue engineering and drug delivery applications. In this work, low-pressure, gaseous CO2 is used as an acidifying agent to fabricate SF hydrogels. This low-pressure CO2 acidification method is compared to an acidification method using high-pressure CO2 to demonstrate the effect of CO2 mass transfer and pressure on SF sol-gel kinetics. The effect of SF molecular weight on the sol-gel kinetics is determined using the low-pressure CO2 method. The results from these studies demonstrate that low-pressure CO2 processing proves to be a facile method for synthesizing 3-D SF hydrogels.
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The uniform and mild acidification environment from CO2 bubbling is more conducive to formation of uniform three-dimensional crosslinked porous hydrogel structure.
“The uniform and mild acidification environment is more conducive to the formation of uniform three-dimensional crosslinked porous hydrogel structure [32].”
The lower pH reduced the gelation time from days to mere hours.
“The lower pH reduced the gelation time from days to mere hours [25].”
From CO2 assisted synthesis of silk fibroin driven robust N-doped carbon aerogels coupled with nickel–cobalt particles as highly active electrocatalysts for HER (2021) · cites this paper as [25]
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Crosslinking strategies for silk fibroin hydrogels: promising biomedical materials
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Kim, H. H., Kim, J. W., Choi, J., Park, Y. H., & Ki, C. S. (2018) · Polymer · doi:10.1016/j.polymer.2018.08.019 · Cited by 53 · see citing works
Gong, S., Xiao, X., Wang, W., Sam, D. K., Lu, R., Xu, Y., Liu, J., Wu, C., & Lv, X. (2021) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2021.05.054 · Cited by 42 · see citing works
Sam, D. K., Wang, W., Gong, S., Sam, E. K., Lv, X., Wang, J., & Liu, J. (2021) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2021.03.243 · Cited by 34 · see citing works
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- Authors
- Mallepally, R. R., Marin, M. A., & McHugh, M. A.
- Affiliations
- Virginia Commonwealth University (US)
- Corresponding author
- Mallepally, Rajendar R.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 10
- Issue
- 10
- Pages
- 4419-4424
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2063569728
- Cited by
- 46
- References
- 0
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 6 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 6 · 2014: 1
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Office of Naval Research (N000140710526)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- silk fibroin, hydrogels, aerogels, low-pressure CO2, high-pressure CO2, CO2 mass transfer, molecular weight effects, tissue engineering scaffolds, drug delivery
- MeSH terms
- Animals, Carbon Dioxide, Fibroins, Molecular Weight, Bombyx, Porosity, Hydrogels
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