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Cited by 16Tissue Engineering and Regenerative MedicineCardiac Ischemia and Reperfusion3D Printing in Biomedical Research

Transcriptome profiling of 3D co-cultured cardiomyocytes and endothelial cells under oxidative stress using a photocrosslinkable hydrogel system

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Yue, Xiaoshan, Acun, Aylin, Zorlutuna, Pinar·2017·Acta Biomaterialia
DOI 10.1016/j.actbio.2017.06.031PMC PMC5563470
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Abstract

Myocardial infarction (MI) is one of the most common among cardiovascular diseases. Endothelial cells (ECs) are considered to have protective effects on cardiomyocytes (CMs) under stress conditions such as MI; however, the paracrine CM-EC crosstalk and the resulting endogenous cellular responses that could contribute to this protective effect are not thoroughly investigated. Here we created biomimetic synthetic tissues containing CMs and human induced pluripotent stem cell (hiPSC)-derived ECs (iECs), which showed improved cell survival compared to single cultures under conditions mimicking the aftermath of MI, and performed high-throughput RNA-sequencing to identify target pathways that could govern CM-iEC crosstalk and the resulting improvement in cell viability. Our results showed that single cultured CMs had different gene expression profiles compared to CMs co-cultured with iECs. More importantly, this gene expression profile was preserved in response to oxidative stress in co-cultured CMs while single cultured CMs showed a significantly different gene expression pattern under stress, suggesting a stabilizing effect of iECs on CMs under oxidative stress conditions. Furthermore, we have validated the in vivo relevance of our engineered model tissues by comparing the changes in the expression levels of several key genes of the encapsulated CMs and iECs with in vivo rat MI model data and clinical data, respectively. We conclude that iECs have protective effects on CMs under oxidative stress through stabilizing mitochondrial complexes, suppressing oxidative phosphorylation pathway and activating pathways such as the drug metabolism-cytochrome P450 pathway, Rap1 signaling pathway, and adrenergic signaling in cardiomyocytes pathway. Statement of significance Heart diseases are the leading cause of death worldwide. Oxidative stress is a common unwanted outcome that especially occurs due to the reperfusion following heart attack or heart surgery. Standard methods of in vivo analysis do not allow dissecting various intermingled parameters, while regular 2D cell culture approaches often fail to provide a biomimetic environment for the physiologically relevant cellular phenotypes. In this research, a systematic genome-wide transcriptome profiling was performed on myocardial cells in a biomimetic 3D hydrogel-based synthetic model tissue, for identifying possible target genes and pathways as protecting regulators against oxidative stress. Identification of such pathways would be very valuable for new strategies during heart disease treatment by reducing the cellular damage due to reperfusion injury.

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  • hCMEC supports efficient metabolic and electrical function by increasing oxygenation and electrophysiological properties through the release of vascular endothelial growth factor (VEGF) and nitric oxide.

    “Moreover hCMEC supports efficient metabolic and electrical function by increasing oxygenation and electrophysiological properties through the release of vascular endothelial growth factor (VEGF) and nitric oxide.[ 55 , 56 ]”

    From Engineered in vitro multi-cell type ventricle model generates long-term pulsatile flow and modulates cardiac output in response to cardioactive drugs (2025) · cites this paper as [55]

    The Introduction (page not provided) describes nitric oxide as an endothelial-cell modulator and says endothelial–cardiomyocyte interactions affect cardiac function and metabolism. The Results and Discussion report that endothelial co-culture stabilizes cardiomyocyte gene expression and metabolism under oxidative stress. The paper does not establish increased oxygenation or electrophysiological properties through these factors; its Introduction identifies cardiomyocytes, not endothelial cells, as releasing VEGF. (LLM, conf=0.86)

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Authors
Yue, X., Acun, A., & Zorlutuna, P.
Affiliations
University of Notre Dame (US)
ORCID
Venue
Acta Biomaterialia
Publisher
Elsevier BV
Published
2017-08
Volume
58
Pages
337-348
ISSN
1742-7061 (ISSN-L)
DOI
10.1016/j.actbio.2017.06.031
PMC
PMC5563470
OpenAlex
W2686400070
Cited by
16
References
2
Citations by year
2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2019: 4 · 2018: 2 · 2017: 4
FWCI
2.80
Type
journal article
Language
en
Open access
Licence
Locations
  • Acta Biomaterialia (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (UL1TR001108)
  • National Center for Advancing Translational Sciences
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
cardiomyocytes, endothelial cells, oxidative stress, myocardial infarction, ischemia-reperfusion injury, 3D co-culture, photocrosslinkable hydrogels, biomimetic tissue engineering, gene expression profiling, oxidative phosphorylation, cytochrome P450 pathway, Rap1 signaling, adrenergic signaling, cell viability
MeSH terms
Animals, Gene Expression Regulation, Rats, Sprague-Dawley, Oxidative Stress, Coculture Techniques, Hydrogels, Gene Expression Profiling, Myocytes, Cardiac, Endothelial Cells, Rats

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