Heterologous expression of phosphite dehydrogenase in the chloroplast or nucleus enables phosphite utilization and genetic selection in picochlorum spp
Open accessAbstract
Microalgae present a path to ameliorate problems associated with climate change via capture and reduction of CO2 to sustainable fuels and chemicals. Picochlorum is a genus of algae recently recognized for potential application in these regards due to its high productivity, thermotolerance, and halotolerance. Foundational genetic tools have recently been established in this genus. However, at present, genetic markers are limited, hindering genetic throughput and trait stacking approaches. To expand the suite of genetic tools and markers available for this genus, we sought to heterologously express the phosphite dehydrogenase (ptxD) gene from Pseudomonas stutzeri WM88 in both the nucleus and chloroplast of Picochlorum renovo and Picochlorum celeri. The resultant strains allow for utilization of phosphite as a sole phosphorous source and as a nuclear and plastidial selection marker for genetic engineering. Growth analysis indicated comparable growth and composition when transgenic algae were grown in media containing phosphite as a sole phosphorus source, as compared to the conventionally used phosphate. Combined, these results expand the genetic toolbox available to the Picochlorum genus and present a potential crop protection and biocontainment strategy.
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Contamination control can be achieved through online monitoring, specialized reactor designs, or innovative genetic systems such as the phosphite dehydrogenase-phosphite system (ptxD-Phi), which allows cultivation using phosphite.
“Contamination control, for instance, can be achieved through online monitoring, specialized reactor designs, or innovative genetic systems such as phosphite dehydrogenase–phosphite system (ptxD–Phi), which allows cultivation using phosphite (Dahlin and Guarnieri, 2022).”
From Microalgae biotechnology and its role in sustainable and healthy food design (2025) · cites this paper as Dahlin and Guarnieri, 2022
The Introduction reports that ptxD enables cultivation on phosphite and that phosphite-based cultivation has reduced contamination in algal and cyanobacterial systems. The paper does not address online monitoring or specialized reactor designs as contamination-control methods. It describes crop protection in Picochlorum as a potential benefit, rather than demonstrating contamination control in that genus. (LLM, conf=0.86)
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References 44 · Cited by 16
- Authors
- Dahlin, L. R., & Guarnieri, M. T.
- Affiliations
- National Laboratory of the Rockies (US)
- Corresponding author
- Guarnieri, Michael T.
- Venue
- Algal Research
- Publisher
- Elsevier BV
- Published
- 2022-03
- Volume
- 62
- Pages
- 102604
- ISSN
- 2211-9264 (ISSN-L)
- OpenAlex
- W4206961596
- Cited by
- 16
- References
- 44
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 4
- Type
- book review
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Algal Research (publishedVersion, cc-by-nc-nd)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- U.S. Department of Energy (DE -AC36-08GO28308, 08GO28308, DE-AC36, AC36‐08GO28308)
- Office of Science (DE-AC36-08GO28308)
- Office of Energy Efficiency and Renewable Energy (DE-AC36-08-GO28308., AC36-08-GO28308, 28812)
- Biological and Environmental Research (DE-AC36-08-GO28308)
- Office of Energy Efficiency
- National Renewable Energy Laboratory (DE-AC36-08-GO28308, AC36-08GO28308)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- phosphite dehydrogenase, Picochlorum, heterologous expression, chloroplast transformation, nuclear transformation, phosphorus sources, transgenic microalgae, biocontainment, crop protection, Pseudomonas stutzeri
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