The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Microplastics cause neurotoxicity, oxidative damage and energy-related changes and interact with the bioaccumulation of mercury in the European seabass, Dicentrarchus labrax (Linnaeus, 1758)
Open accessAbstract
Microplastics pollution is a global paradigm that raises concern in relation to environmental and human health. This study investigated toxic effects of microplastics and mercury in the European seabass (Dicentrarchus labrax), a marine fish widely used as food for humans. A short-term (96 h) laboratory bioassay was done by exposing juvenile fish to microplastics (0.26 and 0.69 mg/L), mercury (0.010 and 0.016 mg/L) and binary mixtures of the two substances using the same concentrations, through test media. Microplastics alone and mercury alone caused neurotoxicity through acetylcholinesterase (AChE) inhibition, increased lipid oxidation (LPO) in brain and muscle, and changed the activities of the energy-related enzymes lactate dehydrogenase (LDH) and isocitrate dehydrogenase (IDH). All the mixtures caused significant inhibition of brain AChE activity (64-76%), and significant increase of LPO levels in brain (2.9-3.4 fold) and muscle (2.2-2.9 fold) but not in a concentration-dependent manner; mixtures containing low and high concentrations of microplastics caused different effects on IDH and LDH activity. Mercury was found to accumulate in the brain and muscle, with bioaccumulation factors of 4-7 and 25-40, respectively. Moreover, in the analysis of mercury concentrations in both tissues, a significant interaction between mercury and microplastics was found. The decay of mercury in the water increased with microplastics concentration, and was higher in the presence of fish than in their absence. Overall, these results indicate that: microplastics influence the bioaccumulation of mercury by D. labrax juveniles; microplastics, mercury and their mixtures (ppb range concentrations) cause neurotoxicity, oxidative stress and damage, and changes in the activities of energy-related enzymes in juveniles of this species; mixtures with the lowest and highest concentrations of their components induced different effects on some biomarkers. These findings and other published in the literature raise concern regarding high level predators and humans consuming fish being exposed to microplastics and heavy metals, and highlight the need of more research on the topic.
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“Previous studies, under experimental conditions, evidenced the harmful effects of MPs ingestion in the brain of sea bass (Dicentrarchus labrax). MPs caused neurotoxicity through acetylcholinesterase (AChE) inhibition and increased lipid oxidation (LPO) [64].”
From Is Wild Marine Biota Affected by Microplastics? (2022) · cites this paper as [64]
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction
Ohkawa, H., Ohishi, N., & Yagi, K. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90738-3 · Cited by 29,746
A new and rapid colorimetric determination of acetylcholinesterase activity
Ellman, G. L., Courtney, K. D., Andres, V., & Featherstone, R. M. (1961) · Biochemical Pharmacology · doi:10.1016/0006-2952(61)90145-9 · Cited by 27,221
Feinleib, M., & Zar, J. H. (1975) · Journal of the American Statistical Association · doi:10.2307/2285423 · Cited by 12,671
Bergmann, M., Gutow, L., & Klages, M. (2015) · Springer eBooks · doi:10.1007/978-3-319-16510-3 · Cited by 3,513
Plastic debris in the open ocean
Cózar, A., Echevarría, F., González-Gordillo, J. I., Irigoien, X., Úbeda, B., Hernández-León, S., Palma, Á. T., Navarro, S., García-de-Lomas, J., Ruiz, A., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1314705111 · Cited by 3,133
Transport and release of chemicals from plastics to the environment and to wildlife
Teuten, E. L., Saquing, J. M., Knappe, D. R. U., Barlaz, M. A., Jonsson, S., Björn, A., Rowland, S. J., Thompson, R. C., Galloway, T. S., Yamashita, R., et al. (2009) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2008.0284 · Cited by 2,938
The impact of debris on marine life
Gall, S. C., & Thompson, R. C. (2015) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2014.12.041 · Cited by 2,187
Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress
Rochman, C. M., Hoh, E., Kurobe, T., & Teh, S. J. (2013) · Scientific Reports · doi:10.1038/srep03263 · Cited by 2,169
A global inventory of small floating plastic debris
van Sebille, E., Wilcox, C., Lebreton, L., Maximenko, N., Hardesty, B. D., van Franeker, J. A., Eriksen, M., Siegel, D., Galgani, F., & Law, K. L. (2015) · Environmental Research Letters · doi:10.1088/1748-9326/10/12/124006 · Cited by 1,713
Research works printed but not linked: 1
- Nasfi, 1995, Total mercury content of sea water on the Tunisian Shore, Fresenius Environ. Bull., 4, 161
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Environmental exposure to microplastics: An overview on possible human health effects
Prata, J. C., da Costa, J. P., Lopes, I., Duarte, A. C., & Rocha-Santos, T. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2019.134455 · Cited by 2,690
Marine microplastic debris: An emerging issue for food security, food safety and human health
Barboza, L. G. A., Vethaak, A. D., Lavorante, B. R. B. D. O., Lundebye, A., & Guilhermino, L. M. (2018) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2018.05.047 · Cited by 1,620
Atmospheric microplastics: A review on current status and perspectives
Zhang, Y., Kang, S., Allen, S., Allen, D., Gao, T., & Sillanpää, M. E. T. (2020) · Earth-Science Reviews · doi:10.1016/j.earscirev.2020.103118 · Cited by 1,457
“Currently, due to their inhalation and combination with other pollutants (mercury or PAHs), microplastics are thought to be an emergent component of air pollution (Barboza et al., 2018; Gasperi et al., 2018; Liu et al., 2019; Rochman et al., 2019; Tourinho et al., 2019; Wright and Kelly, 2017).”
BackgroundA Global Perspective on Microplastics
Hale, R. C., Seeley, M. E., Guardia, M. J. L., Mai, L., & Zeng, E. Y. (2020) · Journal of Geophysical Research Oceans · doi:10.1029/2018jc014719 · Cited by 1,523
“Most such studies examined organic pollutants, but Barboza et al. (2018) evaluated interactions between mercury and microplastics, and resulting toxicological implications in fish.”
BackgroundBarboza, L. G. A., Lopes, C., Oliveira, P., Bessa, F., Otero, V., Henriques, B., Raimundo, J., Caetano, M., Vale, C., & Guilhermino, L. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2019.134625 · Cited by 951
“…et al., 2017) or no significant effect or inhibition (e.g. Oliveira et al., 2013; Avio et al. 2015; Luis et al., 2015; Ribeiro et al., 2017; Barboza et al. 2018c, Ding et al., 2018; Oliveira et al., 2018; Yu et al., 2018), dependending of the species, developmental stage, type of…”
Self-citation
Metadata
- Authors
- Barboza, L. G. A., Vieira, L. R., Branco, V., Figueiredo, N., Carvalho, F., Carvalho, C., & Guilhermino, L.
- Affiliations
- Ministry of Education (BR)
- Universidade do Porto (PT)
- University of Lisbon (PT)
- Corresponding author
- Barboza, Luís Gabriel Antão
- ORCID
- Venue
- Aquatic Toxicology
- Publisher
- Elsevier BV
- Published
- 2018-02
- Volume
- 195
- Pages
- 49-57
- ISSN
- 0166-445X (ISSN-L)
- OpenAlex
- W2779948902
- Cited by
- 766
- References
- 74
- Citations by year
- 2026: 72 · 2025: 97 · 2024: 108 · 2023: 109 · 2022: 129 · 2021: 104 · 2020: 85 · 2019: 44 · 2018: 18
- FWCI
- 18.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Aquatic Toxicology (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- U.S. Environmental Protection Agency
- European Regional Development Fund
- Topics
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Mercury impact and mitigation studies
- Keywords
- microplastics, mercury, Dicentrarchus labrax, neurotoxicity, acetylcholinesterase inhibition, lipid peroxidation, oxidative stress, energy metabolism enzymes, lactate dehydrogenase, isocitrate dehydrogenase, bioaccumulation, mercury bioaccumulation, heavy metals, marine fish
- MeSH terms
- Animals, Bass, Biological Assay, Brain, Energy Metabolism, Environmental Monitoring, Humans, Mercury, Plastics, Water Pollutants, Chemical, Biomarkers, Oxidative Stress
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