Apolipoprotein A-V associates with intrahepatic lipid droplets and influences triglyceride accumulation
Open accessAbstract
Apolipoprotein A-V (apoA-V), secreted solely by the liver, is a low abundance protein that strongly influences plasma triglyceride (TG) levels. In vitro, in transfected hepatoma cell lines apoA-V is largely retained within the cell in association with cytosolic lipid droplets (LD). To evaluate if this is true in vivo, in the present study the amount of apoA-V in the plasma compartment versus liver tissue was determined in APOA5 transgenic (Tg) mice. The majority of total apoA-V ( approximately 80%) was in the plasma compartment. Injection of APOA5 Tg mice with heparin increased plasma apoA-V protein levels by approximately 25% indicating the existence of a heparin-releasable pool. Intrahepatic apoA-V was associated with LD isolated from livers of wild type (WT) and APOA5 Tg mice. Furthermore, livers from APOA5 Tg mice contained significantly higher amounts of TG than livers from WT or apoa5 knockout mice suggesting that apoA-V influences intrahepatic TG levels.
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APOE and APOCIII were identified on nuclear lipid droplets in HepG2 cells, and APOAV has been reported on the surface of cytoplasmic lipid droplets in adipocytes.
“APOE and APOCIII were identified on nuclear LDs in HepG2 cells, and APOAV has previously been reported on the surface of cytoplasmic LDs in adipocytes (Shu et al., 2010; Gao et al., 2012; Sołtysik et al., 2019).”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Shu et al., 2010
The Introduction and Results §3.3 report apoA-V associated with cytosolic lipid droplets in transfected hepatoma cells and mouse liver. They do not place it on droplet surfaces in adipocytes, and the paper does not report APOE or APOCIII on nuclear lipid droplets in HepG2 cells. (LLM, conf=0.86)
Experimental — accuracy not yet measuredAPOV and APOCIII have been shown to go to lipid droplets.
“APOV and APOCIII have been shown to go to LDs (Shu et al., 2010; Gao et al., 2012; Sołtysik et al., 2019).”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Shu et al., 2010
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APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size
Windham, I. A., Powers, A. E., Ragusa, J. V., Wallace, E. D., Zanellati, M. C., Williams, V. H., Wagner, C. H., White, K. K., & Cohen, S. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202305003 · Cited by 96 · see citing works
- Authors
- Shu, X., Nelbach, L., Ryan, R. O., & Forte, T. M.
- Affiliations
- University of California, Berkeley (US)
- Corresponding author
- Forte, Trudy M.
- ORCID
- Ryan, Robert O.
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
- Publisher
- Elsevier BV
- Published
- 2010-05
- Volume
- 1801
- Issue
- 5
- Pages
- 605-608
- ISSN
- 1388-1981 (ISSN-L)
- PMC
- PMC2839894
- OpenAlex
- W2039204216
- Cited by
- 54
- References
- 1
- Citations by year
- 2024: 3 · 2023: 2 · 2022: 3 · 2021: 6 · 2020: 5 · 2019: 5 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 6 · 2014: 1 · 2013: 2 · 2012: 4
- FWCI
- 3.12
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- American Heart Association
- National Heart, Lung, and Blood Institute (HL073061, R01HL073061)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- apolipoprotein A-V, triglyceride accumulation, hepatoma cells, apolipoprotein secretion
- MeSH terms
- Apolipoprotein A-V, Animals, Apolipoproteins, Inclusion Bodies, Heparin, Liver, Male, Mice, Transgenic, Triglycerides, Lipid Metabolism, Mice
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